In vitro study of mouse fibroblast tumor cells with TNF coated and Alexa488 marked silica nanoparticles with an endoscop
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In vitro study of mouse fibroblast tumor cells with TNF coated and Alexa488 marked silica nanoparticles with an endoscopic device for real time cancer visualization Marion Herz1, Andreas Rank1, Günter E. M.Tovar1,2, Thomas Hirth1,2, Dominik Kaltenbacher3, Jan Stallkamp3, Achim Weber1,2 1
Fraunhofer-Institute for Interfacial Engineering and Biotechnology, 2 Institute of Interfacial Engineering, University of Stuttgart, 3 Fraunhofer Institute for Manufacturing Engineering and Automation Nobelstr. 12, 70569 Stuttgart, Germany ABSTRACT Tumor resection done by minimally invasive procedure owns the challenge of a fast and reliable differentiation between healthy and tumorous tissue. We aim at investigating and developing a method for an intraoperative visualization of tumor cells with functionalized nanoparticles. The goal is to use this technique for the intraoperative use. Our so-called biohybrid systems consist of nanoparticles that are produced by Stöber synthesis and coupled with bio active proteins. Such biomimetic nanostructures are capable of imitating the effects of membrane-bound cytokines, which bind to tumor cells for labeling them. A flexible and modular test environment has been developed to evaluate the spraying properties of the particles and to study tissue probes. It enables a fast investigation of different particle configurations and spraying parameters like pressure, spray volume, nozzle geometry, etc.
INTRODUCTION During the course of minimally invasive tumor resection, it is often difficult for the surgeon to clearly distinguish between healthy and tumorous tissue on the endoscopic image. Furthermore, the surgeon does not perceive the haptic and tactile feedback needed to evaluate the tissue by means of palpatation. The use of navigation systems to localize the tumor usually fails due to the presence, quality, accuracy or the informational content of the image data. Therefore a tumor sample is removed during surgery and given to the pathologist. This procedure can take up to half an hour and requires that the operation is “put on hold” for this period of time. However, a final laboratory examination can take significantly longer. To minimize the risk, the surgeon supplements the expertise of the pathologist and a mutual assessment of the tissue status is usually done. This decision is extremely risky, for example, if the partial resection of a tissue or an entire organ depends on it. Quality control of the resection margins is so far not possible by any means. Thus, the necessity of the resection of large volumes remains for reasons of safety. The C-VIS idea concerns with an alternative method which in a short period of time can directly help to visualize the tumor tissue in human body.
EXPERIMENTAL Making tumor tissue visible with nanoparticles The C-VIS method is based on the property of modified nanoparticles attaching to tumor cells, but not to healthy cells. Nanoparticle hybrid systems are synthesized consisting of synthetic nanoparticles and biologically active proteins. For
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