Induced Pluripotent Stem (iPS) Cells Methods and Protocols
This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the
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Kursad Turksen Andras Nagy Editors
Induced Pluripotent Stem (iPS) Cells Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Induced Pluripotent Stem (iPS) Cells Methods and Protocols
Edited by
Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
Andras Nagy Mount Sinai Hospital Samuel Lunenfeld Research Institue Toronto, ON, Canada
Editors Kursad Turksen Ottawa Hospital Research Institute Ottawa, ON, Canada
Andras Nagy Mount Sinai Hospital Samuel Lunenfeld Research Institue Toronto, ON, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3054-8 ISBN 978-1-4939-3055-5 (eBook) DOI 10.1007/978-1-4939-3055-5 Library of Congress Control Number: 2015960271 Springer New York Heidelberg Dordrecht London # Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The demonstration of reprogramming of somatic cells with four transcription factors by Yamanaka’s group in 2006 provided a molecular basis for the earlier results of J. Gurdon published in 1962. These ground-breaking developments have led to a flurry of studies aimed at better understanding many facets of stem cell biology, lineage commitment, and differentiation. The scope and volume of the ongoing work made it timely to put together a series of protocols that can be used by both experts and novices interested in stem cells. We are grateful to all the contributors who agreed to describe their detailed protocols. Without their generosity and input, this volume would not have been possible. One of us [K.T.] would like to acknowledge the outstanding sabbatical s
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