Muscle Stem Cells Methods and Protocols
This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Chapters detail current and updated methods for muscle stem cell isolation, culture, molecular analysis, cellular analysis, and reintroduc
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Eusebio Perdiguero DDW Cornelison Editors
Muscle Stem Cells Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Muscle Stem Cells Methods and Protocols
Edited by
Eusebio Perdiguero Department of Experimental & Health Sciences, University Pompeu Fabra (UPF), Barcelona, Spain
DDW Cornelison Division of Biological Sciences and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA
Editors Eusebio Perdiguero Cell Biology Group Department of Experimental and Health Sciences University Pompeu Fabra (UPF) Barcelona, Spain
DDW Cornelison Division of Biological Sciences and Christopher S. Bond Life Sciences Center University of Missouri Columbia, MO, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6769-8 ISBN 978-1-4939-6771-1 (eBook) DOI 10.1007/978-1-4939-6771-1 Library of Congress Control Number: 2016962638 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface In the last decade, skeletal muscle stem cells have joined hematopoietic, intestinal, and skin stem cells as a standard model to study stem cell function in healthy and aging tissue and the role of stem cells in tissue-specific diseases. Muscle Stem Cells: Methods and Protocols provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Protocols representing current and updated methods for muscle stem cell isola
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