Influence of Mycorrhization on the Growth and Fructan Production in Micropropagated Agave grijalvensis (B. Ullrich) Plan
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RESEARCH ARTICLE
Influence of Mycorrhization on the Growth and Fructan Production in Micropropagated Agave grijalvensis (B. Ullrich) Plantlets Jose´ A. Santiz-Go´mez1 • Reiner Rinco´n-Rosales1 • Miguel Abud-Archila1 • Victor M. Ruı´z-Valdiviezo1 • Federico A. Gutie´rrez-Miceli1 • Luc Dendooven2 • Vianey Mendez-Trujillo3 • Ludwi Rodrı´guez-Hernandez3 • Daniel Gonzalez-Mendoza4
Received: 8 January 2019 / Revised: 24 April 2019 / Accepted: 20 May 2019 Ó The National Academy of Sciences, India 2019
Abstract Agave grijalvensis (B. Ullrich) was inoculated with Glomus fasciculatum (Thaxter sensu Gerd.) Gerd. and Trappe) and fertilized with different amounts of phosphorus (P) during plant development. Mycorrhizal colonization, fructan accumulation and fructosyltransferases enzymatic activities were determined. Glomus fasciculatum increased fresh weight of A. grijalvensis, the number of leaves, root length and fructan concentrations in stems and leaves. Phosphorus increased sucrose content, but reduced glucose and fructose concentrations in both leaves and stems. Activities of 1-fructosyltransferase (1-SST) (EC 2.4.1.99) and b-fructan-1-fructosyltransferase (1-FFT) (EC 2.4.1.100) were determined and explained due to different levels of fructans detected in A. grijalvensis plantlets.
Significance Statement Agave grijalvensis has been recognized as an endemic plant from Mexico with high risk of extinction. The aim of this work was to evaluate the effect of Glomus fasciculatum and phosphorus on the growth of the plantlets of A. grijalvensis and their effects in concentration of fructans which are important for alcoholic fermentation of beverages. & Daniel Gonzalez-Mendoza [email protected] 1
Tecnolo´gico Nacional de Me´xico, Instituto Tecnolo´gico de Tuxtla Gutie´rrez, Carretera Panamericana Km 1080, 29020 Tuxtla Gutie´rrez, Chiapas, Mexico
2
Laboratory of Soil Ecology, Centro de Investigacio´n y de Estudios Avanzados del Instituto Polite´cnico Nacional (CINVESTAV),, Av. IPN 2508, 07360 Me´xico, D.F., Mexico
3
Tecnolo´gico Nacional de Me´xico, Instituto Tecnolo´gico Superior de Cintalapa, Carretera Panamericana Km 995, 30400 Cintalapa, Chiapas, Mexico
4
Instituto de Ciencias Agrı´colas de LA Universidad Auto´noma de Baja California (ICA-UABC), Carretera a Delta s/n C.P. 21705, Ejido Nuevo Leo´n, Mexicali, Baja California, Mexico
Keywords Enzymatic activities Fructosyltransferases Inulin Glomus fasciculatum
Introduction Mexico has been considered the center of origin and biodiversity of the genus Agave due to the taxonomic diversity within its territory. Actually, 272 of 310 species have been found in Mexico [1]. Agave species are resources of arid zones and semiarid regions of Mexico with great relevance for the alcoholic beverage industry and natural fibers [2, 3]. Members of the Agavaceae family are distributed and well adapted to arid and semiarid regions. Particularly, Agave grijalvensis B. Ullrich is an endemic plant from Chiapas in the southeast Mexico. According to the Official Mexican Standard NOM-
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