Inheritance and metabolomics of the resistance of two F 2 populations of Phaseolus spp. to Acanthoscelides obtectus
- PDF / 1,496,000 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 4 Downloads / 148 Views
ORIGINAL PAPER
Inheritance and metabolomics of the resistance of two F2 populations of Phaseolus spp. to Acanthoscelides obtectus José Cruz Jiménez‑Galindo1,2 · María Tortosa1 · Pablo Velasco1 · María De La Fuente1 · Bernardo Ordás1 · Rosa Ana Malvar1 Received: 21 February 2020 / Accepted: 11 August 2020 © Springer Nature B.V. 2020
Abstract The bean weevil Acanthoscelides obtectus is one of the major storage pests of dry bean. Tepary bean (Phaseolus acutifolius) is a great source of resistance against A. obtectus to improve dry bean production. We developed two F2 populations of Phaseolus spp. by crossing bean genotypes resistant to A. obtectus × susceptible, to (1) search for metabolites related to bean weevil resistance using an untargeted metabolomics approach and (2) understand the genetics of resistance to A. obtectus attack using the number of adults emerged from seeds as resistance criteria. The segregation of the number of adults emerged from seeds showed that the genetics of resistance (0 emerged adults = resistant, 1 or more emerged adults = susceptible) was controlled by a single dominant gene in two (resistant × susceptible) F 2 populations. From nine metabolites detected, pipecolic acid concentration was significantly higher in the testa of resistant individuals than in the testa of susceptible individuals. From a breeding point of view, (P. Saltillo × T. amarillo) F2 is of special interest because it is derived from a cultivated tepary × cultivated common bean cross and showed 3:1 resistant vs susceptible segregation. Therefore, this F 2 is a suitable base material for breeding programmes to obtain new bean lines with good agronomic performance, seed quality and resistance to insect attack. Pipecolic acid plays a role in resistance against A. obtectus. Keywords Seed coat resistance · Pipecolic acid · Acanthoscelides obtectus · Phaseolus vulgaris · Phaseolus acutifolius
Introduction Common bean (Phaseolus vulgaris L.) is the most important legume for direct consumption in the world and is an important source of protein in the diet of many developing countries. The total area of P. vulgaris L. harvested in 2006–2008 was 26.67 million hectares, with a total production of 18.82 million tons globally (Akibode and Maredia 2012). Post-harvesting damage and losses are mainly caused by two bruchid species, A. obtectus and Zabrotes subfasciatus, with damages of approximately 7–40% (Mbogo et al.
* José Cruz Jiménez‑Galindo [email protected]; [email protected] 1
Misión Biológica de Galicia, Spanish National Research Council (CSIC), 36080 Apartado 28, Pontevedra, Spain
Sitio Experimental Sierra de Chihuahua, National Institute of Forestry Agriculture and Livestock Research (INIFAP), Ave. Hidalgo 1213, Cd. Cuauhtémoc, 31500 Chihuahua, Mexico
2
2009), which implies a loss of 1.95–11.16 million tons worldwide per year. Acanthoscelides obtectus is a devastating insect capable of causing severe bean crop losses in widespread regions of Latin America, Africa, the United States, and E
Data Loading...
