Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chi
- PDF / 1,764,062 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 67 Downloads / 153 Views
VETERINARY MICROBIOLOGY - RESEARCH PAPER
Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens Fernanda de Oliveira Barbosa 1 & Oliveiro Caetano de Freitas Neto 2 & Lucas Bocchini Rodrigues Alves 1 & Valdinete Pereira Benevides 1 & Andrei Itajahy Secundo de Souza 1 & Marcela da Silva Rubio 1 & Adriana Maria de Almeida 1 & Mauro Mesquita Saraiva 1 & Celso José Bruno de Oliveira 3 & John Elmerdahl Olsen 4 & Angelo Berchieri Junior 1 Received: 13 May 2020 / Accepted: 27 October 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Salmonella Enteritidis causes infections in humans and animals which are often associated with extensive gut colonization and bacterial shedding in faeces. The natural presence of flagella in Salmonella enterica has been shown to be enough to induce proinflammatory responses in the gut, resulting in recruitment of polymorphonuclear cells, gut inflammation and, consequently, reducing the severity of systemic infection in chickens. On the other hand, the absence of flagellin in some Salmonella strains favours systemic infection as a result of the poor intestinal inflammatory responses elicited. The hypothesis that higher production of flagellin by certain Salmonella enterica strains could lead to an even more immunogenic and less pathogenic strain for chickens was here investigated. In the present study, a Salmonella Enteritidis mutant strain harbouring deletions in clpP and fliD genes (SE ΔclpPfliD), which lead to overexpression of flagellin, was generated, and its immunogenicity and pathogenicity were comparatively assessed to the wild type in chickens. Our results showed that SE ΔclpPfliD elicited more intense immune responses in the gut during early stages of infection than the wild type did, and that this correlated with earlier intestinal and systemic clearance of the bacterium. Keywords Flagellin . Poultry . Salmonellosis . Mutant . Deletion
Introduction Responsible Editor: Waldir P. Elias Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s42770-02000399-7. * Oliveiro Caetano de Freitas Neto [email protected] 1
Department of Veterinary Pathology from the School of Agriculture and Veterinarian Sciences (FCAV), Jaboticabal, State University of São Paulo, Jaboticabal, Brazil
2
Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais (UFMG), Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil
3
Department of Animal Science, Center for Agricultural Sciences, Federal University of Paraiba (CCA/UFPB), Areia, PB, Brazil
4
Department of Veterinary and Animal Sciences, University of Copenhagen, Stigbøjlen 4, 1870 Frederiksberg C, Denmark
Salmonella are Gram-negative, rod-shaped bacteria that belong to the Enterobacteriaceae family. Currently, there are about 2659 recognized serovars, most of which are capable of causing illnesses in both humans and animals [1]. Among them, only
Data Loading...
