Integration of a microfluidic system into a conventional luminescence detector using a 3D printed alignment device
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ORIGINAL PAPER
Integration of a microfluidic system into a conventional luminescence detector using a 3D printed alignment device Ángela Écija-Arenas 1
&
Vanesa Román-Pizarro 1
&
Juan Manuel Fernández-Romero 1
Received: 3 September 2020 / Accepted: 7 October 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A useful 3D printed device for the inside microfluidic integration into a conventional optical detector has been developed. The coupling system supposes the complete integration of a microfluidic device inside the sample compartment of a conventional spectrofluorimeter. For this purpose, a commercial chip-holder, including a microfluidic chip, was anchored inside the detector using a “lab-built” 3D printing alignment prototype. The variables affecting the position of the 3D printed device, such as horizontal and vertical and rotary angles, were optimized. The usefulness of the microfluidic integration system has been tested using an organized suspension of separated hybrid magnetoliposomes containing nanomaterials that were previously separated using a multiphase density gradient centrifugation (MDGC) method. The whole integration system consists of three well-established parts: the impulsion unit, the displacement unit, and the microfluidic chip. The impulsion unit is formed by two syringe pumps, which propel under microflow-rate regime the solutions through to the microfluidic system. The first fluid incorporates an immiscible solution that provides the solution which fills positive oil/water (O/W) displacement unit. In this unit, the previously organized MDGC suspension, which includes different liposome populations, was layer-by-layer displaced to a y-mixer microfluidic chip. The separation content merges with the second solution propelled by the other syringe pump. This solution incorporates a surfactant that promotes the liposome lysis. The novelty supposes the easy incorporation of a 3D printer alignment device, which facilitates the incorporation of the microfluidic channel focused into the optical pathway of the luminescence detector. Keywords Integrated microfluidic/luminescence detector . 3D printing alignment device . Multidensity gradient centrifugation (MDGC) . Hybrid magnetoliposomes Highlights • The use of a 3D printed device is proposed for integrating microfluidic systems with conventional optical detectors. • Materials were separated by a multiphase density gradient centrifugation (MDGC) system. • A positive O/W displacement unit transfers the vial content into the integrated system. • The system utility was demonstrated monitoring different MDGC separated materials. • Hybrid magnetoliposomes with Fe3O4@AuNPs-DT and different fluorophores were studied. * Juan Manuel Fernández-Romero [email protected] Ángela Écija-Arenas [email protected] Vanesa Román-Pizarro [email protected] 1
Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina y Nanoquímica (IUNAN), Universidad de Córdoba, Campus de Rabanales, Edificio Anexo “Marie Curie”, 14
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