Interactive role of zinc and iron lysine on Spinacia oleracea L. growth, photosynthesis and antioxidant capacity irrigat

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RESEARCH ARTICLE

Interactive role of zinc and iron lysine on Spinacia oleracea L. growth, photosynthesis and antioxidant capacity irrigated with tannery wastewater Ihsan Elahi Zaheer1 • Shafaqat Ali1,2 • Muhammad Hamzah Saleem3 • Mohsin Ali4 • Muhammad Riaz5 • Sehar Javed6 • Anam Sehar1 • Zohaib Abbas1 Muhammad Rizwan1 • Mohamed A. El-Sheikh7 • Mohammed Nasser Alyemeni7



Received: 21 September 2020 / Revised: 10 October 2020 / Accepted: 12 November 2020  Prof. H.S. Srivastava Foundation for Science and Society 2020

Abstract Untreated wastewater contains toxic amounts of heavy metals such as chromium (Cr), which poses a serious threat to the growth and physiology of plants when used in irrigation. Though, Cr is among the most widespread toxic trace elements found in agricultural soils due to various anthropogenic activities. To explore the interactive effects of micronutrients with amino acid chelators [iron-lysine (Fe-lys) and zinc-lysine (Zn-lys)], pot experiments were conducted in a controlled environment, using spinach (Spinacia oleracea L.) Ihsan Elahi Zaheer and Muhammad Hamzah Saleem have contributed equally to this work. & Shafaqat Ali [email protected] & Muhammad Hamzah Saleem [email protected] Ihsan Elahi Zaheer [email protected]

plant irrigated with tannery wastewater. S. oleracea was treated without Fe and Zn-lys (0 mg/L Zn-lys and 0 mg/L Fe-lys) and also treated with various combinations of (interactive application) Fe and Zn-lys (10 mg/L Zn-lys and 5 mg/L Fe-lys), when cultivated at different levels [0 (control) 33, 66 and 100%) of tannery wastewater in the soil having a toxic level of Cr in it. According to the results, we have found that, high concentration of Cr in the soil significantly (P \ 0.05) reduced plant height, fresh biomass of roots and leaves, dry biomass of roots and leaves, root length, number of leaves, leaf area, total chlorophyll contents, carotenoid contents, transpiration rate (E), stomatal conductance (gs), net photosynthesis (PN), and water 1

Department of Environmental Sciences and Engineering, Government College University, Allama Iqbal Road, Faisalabad 38000, Pakistan

2

Department of Biological Sciences and Technology, China Medical University, Taichung 40402, Taiwan

3

MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

4

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100000, China

5

Root Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, Guangdong, China

6

Department of Botany, University of Punjab, Lahore, Pakistan

7

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Mohsin Ali [email protected] Muhammad Riaz [email protected] Sehar Javed [email protected] Anam Sehar anamsehar.lcwu