Intracellular Lipid Transport Methods and Protocols
This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long dis
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Guillaume Drin Editor
Intracellular Lipid Transport Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Intracellular Lipid Transport Methods and Protocols
Edited by
Guillaume Drin Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur and CNRS, Valbonne, France
Editor Guillaume Drin Institut de Pharmacologie Mole´culaire et Cellulaire Universite´ Coˆte d’Azur and CNRS Valbonne, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9135-8 ISBN 978-1-4939-9136-5 (eBook) https://doi.org/10.1007/978-1-4939-9136-5 Library of Congress Control Number: 2019931902 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The boundaries and complex architecture of cells rely on membranes that result from the assembly of many lipids, along with proteins. Lipid chemistry is highly diverse with thousands of lipid species identified to date. Inside prokaryotic and eukaryotic cells, lipid species are not randomly distributed between membranes, quite the contrary. In actuality, membranes are endowed with precise physicochemical features and selectively host particular lipids with signaling roles. For instance, it is well described that, in eukaryotic cells, each organelle membrane has its own molecular identity, which is essential for many proteins to function properly and support key functions. At any time, underlying mechanisms adjust and m
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