Glucose Transport Methods and Protocols
This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression s
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Karin Lindkvist-Petersson Jesper S. Hansen Editors
Glucose Transport Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Glucose Transport Methods and Protocols
Edited by
Karin Lindkvist-Petersson Department of Experimental Medical Science, Lund University, Lund, Sweden
Jesper S. Hansen Department of Experimental Medical Science, Lund University, Lund, Sweden
Editors Karin Lindkvist-Petersson Department of Experimental Medical Science Lund University Lund, Sweden
Jesper S. Hansen Department of Experimental Medical Science Lund University Lund, Sweden
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7506-8 ISBN 978-1-4939-7507-5 (eBook) https://doi.org/10.1007/978-1-4939-7507-5 Library of Congress Control Number: 2017959607 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover caption: Front cover artwork by Nieng Yan Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Glucose is one of the main energy sources for living organisms, and undoubtedly one of the most important compounds to life. In our body, glucose is the preferred energy resource for most cells. Mechanisms have consequently evolved to regulate its levels in the body. The first and limiting step in glucose metabolism is its transport across the plasma membrane. In eukaryotic cells, this transport is mediated by members of the GLUT protein family that are encoded by the SLC2A genes. They belong to the major facilitator superfami
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