Investigation of Physicochemical and Cytotoxic Potential of Ocimum basilicum Leaf Extract Mediated Magnetite Nanoparticl
- PDF / 1,843,011 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 16 Downloads / 204 Views
(0123456789().,-volV)(0123456789().,-volV)
ORIGINAL PAPER
Investigation of Physicochemical and Cytotoxic Potential of Ocimum basilicum Leaf Extract Mediated Magnetite Nanoparticles: In Vitro Gulshan Dhillon1 • Naveen Kumar2,3 • Mansi Chitkara1 • Naveed Pervaiz4 • Ravinder Kumar4 Inderjeet Singh Sandhu1
•
Received: 6 August 2020 / Accepted: 12 October 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In this report, an environment friendly and cost effective green synthesis method of magnetite nanoparticles (Fe3O4 NPs) was introduced. Fe3O4 NPs were successfully synthesized by green route using Ocimum basilicum leaf extract. The structural investigation of Fe3O4 NPs using X-Ray diffraction technique revealed the cubic crystallinity with Fd3m space group, which was confirmed with an aid of Rietveld refinement. No secondary phases (such as Fe2O3 and FeO) were observed, which confirmed the high purity of synthesized Fe3O4 NPs. The characteristics peak at 3320 cm-1 displayed the phenolic constituents of Ocimum basilicum leaf extract and peak at 665 cm-1 illustrated the Fe–O bending, which confirmed the formation of Fe3O4 NPs. Ocimum basilicum leaf extract mediated Fe3O4 NPs possess spherical morphology with an average particle size * 15 nm as depicted by transmission electron microscopy (TEM). The magnetic potential of Ocimum basilicum leaf extract mediated Fe3O4 NPs was also carried out by using a vibration sample magnetometer (VSM), which revealed the superparamagnetic behavior of Fe3O4 NPs. Cytotoxicity of the as-synthesized Fe3O4 NPs nanoparticles was also examined on the human breast cancer cell line, which illustrated the efficacy of the synthesized nanoparticles with a low value of IC50 (* 17.75 lg/ml). The results displayed the potent anticancerous effect of Ocimum basilicum leaf extract capped Fe3O4 NPs, which made these nanoparticles suitable for a drug-loaded vehicle for the drug delivery system for biomedical applications. Keywords Fe3O4 NPs Ocimum basilicum Magnetism In vitro
Highlights 1. Biosynthesis of Ocimum basilicum leaf extract mediated Fe3O4 NPs. 2. As-synthesized nanoparticles are found to be spherical with an average size of * 15 nm.
3. A non-toxic, environmentally benign, and cost-effective method is introduced. 4. The potent cytotoxic potential has been observed on the human breast cancer cell line.
Introduction & Inderjeet Singh Sandhu [email protected] 1
Chitkara University Institute of Engineering and Technology, Chitkara University, Punjab, India
2
Department of Physics, Sri Guru Gobind Singh College, Panjab University, Chandigarh 160026, India
3
Department of Applied Sciences, Punjab Engineering College (Deemed to be University), Chandigarh 160012, India
4
Department of Zoology, Panjab University, Chandigarh 160014, India
In recent years, magnetic iron oxide nanoparticles have shown great potential due to a wide range of applications including environment remediation [1], ferrofluid [2], catalysts [3], magnetic storage media [4]
Data Loading...
