Isolation and characterization of thirteen novel dinucleotide microsatellite loci from Tetraena mongolica Maxim
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TECHNICAL NOTE
Isolation and characterization of thirteen novel dinucleotide microsatellite loci from Tetraena mongolica Maxim YingBiao Zhi • Ling Ding • Yupeng Wang Longfei Hu • Yupeng Hua • Kai Zhao • Jiaqing He • Chi Yang • Baowei Zhang
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Received: 1 November 2013 / Accepted: 25 December 2013 Ó Springer Science+Business Media Dordrecht 2014
Abstract We developed 13 polymorphic microsatellite loci isolated from Tetraena mongolica genomic libraries, from a small area in western Inner Mongolia, China. Polymorphisms detected in 18 individuals ranged from 3 to 18 alleles per locus. The observed and expected heterozygosity ranged from 0.444 to 0.944 and 0.586 to 0.948, respectively. The polymorphic information content value ranged from about 0.505 to 0.916. Three loci showed significant deviations from the Hardy–Weinberg equilibrium, while we did not detect any linkage between loci. These markers show high levels of polymorphism, and are of use for future studies.
YingBiao Zhi and Ling Ding have contributed equally to this article. Y. Zhi College of Environment and Resources, Inner Mongolia University, Hohhot 010021, Inner Mongolia, China Y. Zhi Y. Hua College of Ordos, Inner Mongolia University, Ordos 017000, Inner Mongolia, China L. Ding L. Hu J. He B. Zhang (&) School of Life Science, Anhui University, Hefei 230039, Anhui, China e-mail: [email protected]; [email protected] Y. Wang College of Resources and Environment, Anhui Agriculture University, Hefei 230039, Anhui, China K. Zhao School of Life Science, Anqing Normal University, Anqing 246000, Anhui, China C. Yang College of Life Science, Inner Mongolia University, Huhhot 010021, Inner Mongolia, China
Keywords Tetraena mongolica Microsatellite Dinucleotide Genetic diversity
Tetraena mongolica Maxim, is a narrowly monotypic genus of the Zygophyllaceae family, and is endemic to the western part of Inner Mongolia, China (Zhang and Yang 2000). This plant plays a critical role as a windbreak and stabilizing the soil. However, the habitat range of T. mongolica has been declining at an alarming rate because of human overexploitation (Zhang and Yang 2000). Furthermore, recent research has shown that this endemic and geographically restricted species has difficulty setting seed (Wang et al. 2000). This phenomenon leads to lower genetic differentiation, based on allozyme and SSR data (Zhang and Yang 2000). Therefore, it is important to establish effective and efficient conservation practices for T. mongolica. It is also necessary to understand the genetic diversity of T. mongolica to develop appropriate conservation strategies. In recent years, the screening of molecular markers has become a popular way to assess genetic diversity. In this study, we describe the isolation and characterization of 13 microsatellite markers in T. mongolica that are expected to be of use in future studies, of population structure, and gene flow patterns. Leaf tissue was ground to powder in FastPre 120 (MP Biomedicals). Genomic DNA was extracted using a modified version o
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