Isolation and characterization of a tetramethylammonium-degrading Methanococcoides strain and a novel glycine betaine-ut
- PDF / 625,452 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 93 Downloads / 175 Views
ORIGINAL PAPER
Isolation and characterization of a tetramethylammoniumdegrading Methanococcoides strain and a novel glycine betaineutilizing Methanolobus strain Tomislav Ticak · Dinesh Hariraju · Margarette Bayron Arcelay · Brock A. Arivett · Steven E. Fiester · Donald J. Ferguson Jr.
Received: 7 August 2014 / Revised: 23 September 2014 / Accepted: 27 September 2014 / Published online: 16 October 2014 © Springer-Verlag Berlin Heidelberg 2014
Abstract Two novel strains of methanogens were isolated from an estuarine sediment with the capability to utilize quaternary amines. Based on the 16S rRNA analysis, strain B1d shared 99 % sequence identity with Methanolobus vulcani PL-12/MT and strain Q3c shared 99 % identity with Methanococcoides sp. PM1 and PM2, but our current isolates display clearly different capabilities of growth on quaternary amines and were isolated based on these capabilities. Strain Q3c was capable of growth on tetramethylammonium and choline, while strain B1d was capable of growth on glycine betaine. Ml. vulcani PL-12/MT was incapable of growth on glycine betaine, indicating an obvious distinction between strains B1d and PL-12/MT. Strain Q3c now represents the only known tetramethylammonium-utilizing methanogen in isolation. Strain B1d is the first quaternary amine-utilizing methanogen from the genus Methanolobus. This study suggests that quaternary amines may
Communicated by Harald Huber. Electronic supplementary material The online version of this article (doi:10.1007/s00203-014-1043-6) contains supplementary material, which is available to authorized users. T. Ticak · D. Hariraju · B. A. Arivett · S. E. Fiester · D. J. Ferguson Jr. (*) Department of Microbiology, Miami University, 700 East High Street, Oxford, OH 45056, USA e-mail: [email protected] M. B. Arcelay Department of Biology, University of Puerto Rico Mayagüez, 259 Alfonso Valdez Blvd., Mayagüez 00680, Puerto Rico D. J. Ferguson Jr. Miami University Hamilton, 1601 University Boulevard, Hamilton, OH 45011, USA
serve as ready precursors of biological methane production in marine environments. Keywords Methanogen · Glycine betaine · Tetramethylammonium · Choline · Methanococcoides · Methanolobus
Introduction Biological methane production, or methanogenesis, is a topic of considerable global and environmental interest due to the potential of methane to serve as a renewable energy source and its potency as a greenhouse gas (Rothman et al. 2014). Canonical methanogenesis is specific to the domain Archaea, and by studying methanogenesis, we can better understand global carbon and nitrogen cycling. Furthermore, studies related to quaternary amine degradation will give insights into the remediation of toxic compounds, i.e., tetramethylammonium (Tanaka 1994; Asakawa et al. 1998), or cardiovascular associated health factors such as choline and glycine betaine (Wang et al. 2011; Tang et al. 2013; Wang et al. 2014). Methanogenesis encompasses three defined pathways: hydrogenotrophic, aceticlastic, and methylotrophic (Thauer et al. 2008)
Data Loading...
