Ligninolytic enzyme involved in removal of high molecular weight polycyclic aromatic hydrocarbons by Fusarium strain ZH-
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RESEARCH ARTICLE
Ligninolytic enzyme involved in removal of high molecular weight polycyclic aromatic hydrocarbons by Fusarium strain ZH-H2 Xiaoxue Zhang 1 & Xiaomin Wang 1 & Cheng Li 1 & Lixiu Zhang 1 & Guohui Ning 1 & Wei Shi 1 & Xuena Zhang 2 & Zhixin Yang 1,3 Received: 29 January 2020 / Accepted: 20 July 2020 # The Author(s) 2020
Abstract The capacity of Fusarium sp. strain ZH-H2 to secret lignin peroxidase (LiP), laccase (Lac), and manganese peroxidase (MnP) and degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) was studied. When the fungus was grown in control mineral salt medium for 4 days, LiP and Lac activities were detected at 8871 U L−1 and 5123 U L−1, respectively. In the presence of HMW-PAHs as the sole carbon source, only LiP activity was detectable, and LiP activity had significantly reduced HMW-PAHs at day 7, with a maximum decrease of 85.9%. A strong correlation between LiP activity and HMW-PAHs removal efficiency could be fit into various models, with the highest correlation coefficients obtained for quadratic functions (P < 0.01). When a specific enzyme inhibitor was added, the ability of Fusarium to remove HMW-PAHs was reduced from 85.9 to 66.7%, depending on the inhibitor’s concentration. Meanwhile, the determined activity of LiP was reduced from 11.4 to 48.6%. We conclude that in the presence of HMW-PAHs as the only carbon source to support growth, Fusarium ZH-H2 mainly produces LiP but not Lac or MnP for HMW-PAHs degradation. To our knowledge, it was the first time to propose a metabolic lignin peroxidase characterization of HMW-PAHs degradation by Fusarium sp. strains. Keywords Fusarium ZH-H2 . HMW-PAHs . Biodegradation . Ligninolytic system . Laccase . Lignin peroxidase
Introduction Polycyclic aromatic hydrocarbons (PAHs) are organic pollutants that are persistent in the environment with carcinogenic, mutagenic, and teratogenic properties (Meudec et al. 2007,Yuchun 2008). High molecular weight PAHs (HMWPAHs) are compounds with four or more benzene rings, and Xiaoxue Zhang and Xiaomin Wang contributed equally to this study and share first authorship. Responsible Editor: Robert Duran * Zhixin Yang [email protected] 1
College of Resource and Environmental Sciences, Agricultural University of Hebei, 2596 Lekai South Street, Baoding 0710001, People’s Republic of China
2
TianJin Xiqing Economic-Technological Development Area, Tianjin 0710001, People’s Republic of China
3
Key Laboratory for Farmland Eco-Environment, Hebei Province, Agricultural University of Hebei, Baoding, People’s Republic of China
their liposolubility generally increases, and water solubility reduces with increasing numbers of these rings. Due to their chemical characteristics, HMW-PAHs are particularly persistent and can accumulate in the food chain, posing serious threats to human health and the environment (Mastrangelo et al. 1996). Microbial biodegradation of waste material containing HMW-PAHs is an effective approach to degrade these pollutants, as it combines high efficiency and sustainabi
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