LncRNA DANCR regulates the growth and metastasis of oral squamous cell carcinoma cells via altering miR-216a-5p expressi
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RESEARCH ARTICLE
LncRNA DANCR regulates the growth and metastasis of oral squamous cell carcinoma cells via altering miR‑216a‑5p expression Xing‑Hui Qu1 · You‑Ling Shi1 · Yan Ma1 · Wei‑Wei Bao1 · Lei Yang1 · Jin‑Chao Li2 · Fan Zhang3 Received: 13 February 2020 / Accepted: 5 August 2020 © Japan Human Cell Society 2020
Abstract The study aims to investigate how DANCR can alter the growth and metastasis of oral squamous cell carcinoma (OSCC) cells by regulating miR-216a-5p. The expression of DANCR and miR-216a-5p in OSCC patients and cells were measured. SCC15 and CAL-27 cells were selected to divide into Control, sh-NC, DANCR shRNA, DANCR, miR-216a-5p mimic, and DANCR + miR-216a-5p mimic groups. Dual-luciferase reporter gene assay was performed for the verification of the targeting relationship between miR-216a-5p and DANCR/Bcl-2/KLF12. We also quantified the abilities of OSCC cells regarding proliferation, invasion, migration and apoptosis, and the expression levels of apoptosis-related proteins were measured. Finally, the tumor-bearing nude mice were established to verify the effect of DANCR in vivo. Up-regulated DANCR expression and down-regulated miR-216a-5p expression were observed in both OSCC tissues and cells, and they were proven strongly correlated to the histological grade, clinical staging and lymph node metastasis of OSCC patients. Dual-luciferase reporter gene assay showed a target relationship between DANCR and miR-216a-5p, as well as between miR-216a-5p and Bcl-2/ KLF12. Both DANCR shRNA and miR-216a-5p mimic decreased proliferative, migration and invasive abilities of OSCC cells with increased cell apoptosis. However, DANCR group showed completely opposite trends. Moreover, miR-216a-5p mimic could reverse the role of DANCR in promoting tumor growth. In-vivo experiment confirmed the inhibitory role of DANCR shRNA in tumor growth and metastasis. We concluded that DANCR may promote the growth and metastasis of OSCC cells and suppress OSCC cell apoptosis by sponging miR-216a-5p. Keywords DANCR · miR-216a-5p · Oral squamous cell carcinoma · Bcl-2 · KLF12
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13577-020-00411-0) contains supplementary material, which is available to authorized users. * Fan Zhang [email protected] 1
Department of Orthodontics, Dongfeng Stomatological Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China
2
Department of Oral Surgery, Dongfeng Stomatological Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China
3
Department of Pediatric Dentistry, Dongfeng Stomatological Hospital, Hubei University of Medicine, No. 16, Daling Road, Zhangwan District, Shiyan 442000, Hubei Province, China
Oral squamous cell carcinoma (OSCC) takes up approximately 90% of all head and neck cancers and its high morbidity pose a grave threat to human health and life quality [1]. In recent years, there are over 300,000 newly diagnosed OSCC patients worldwide each year with the annua
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