LncRNA NCK1-AS1 in plasma distinguishes oral ulcer from early-stage oral squamous cell carcinoma
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(2020) 27:16 Le et al. J of Biol Res-Thessaloniki https://doi.org/10.1186/s40709-020-00126-1
Open Access
RESEARCH
LncRNA NCK1‑AS1 in plasma distinguishes oral ulcer from early‑stage oral squamous cell carcinoma Fei Le1†, Yangqian Ou2†, Ping Luo3 and Xiaoming Zhong4*
Abstract Background: Oral squamous cell carcinoma (OSCC) at early stages can be misdiagnosed as an oral ulcer (OU) due to similar symptoms, such as chronic and indurated ulcer. LncRNA NCK1-AS1 has been characterized as a key player in cervical cancer, while its role in OSCC is unknown. Methods: All participants were selected at Jiangxi Province Tumor Hospital from December 2016 to December 2018. Expression levels of NCK1-AS1 and miR-100 in plasma from both OSCC and OU patients were measured by RT-qPCR. Diagnostic analysis was performed through ROC curve. Potential interactions between NCK1-AS1 and miR-100 were detected by cell transfection experiments. Cell invasion and migration were assessed by Transwell assays. Results: The expression of NCK1-AS1 was upregulated in early-stage OSCC patients but not in OU patients. Upregulation of NCK1-AS1 distinguished OSCC patients from OU patients. The expression of miR-100 was inversely correlated with the expression of NCK1-AS1. Overexpression of NCK1-AS1 was followed by promoted OSCC cell invasion and migration. Overexpression of miR-100 did not affect the expression of NCK1-AS1 but inhibited the role of NCK1-AS1. Conclusions: Therefore, NCK1-AS1 may promote the metastasis of OSCC by downregulating miR-100. Keywords: Oral squamous cell carcinoma, Oral ulcer, lncRNA NCK1-AS1, miR-100 Background Oral squamous cell carcinoma (OSCC) accounts for more than 80% of head and neck cancer, which is a common malignancy in clinical practices [1, 2]. Smoking, alcohol abuse, betel quid chewing consumption and HPV infections have been identified as the major risk factors for OSCC [3]. However, the molecular mechanism of OSCC pathogenesis remains unclear. Although efforts have been made to develop an effective treatment of OSCC, mortality rate of OSCC patients is still high, with an overall 5 year survival rate lower than 50% [4]. *Correspondence: [email protected] † Fei Le and Yangqian Ou contributed equally to this work 4 Department of Radiotherapy, Jiangxi Province Tumor Hospital, No.519 Beijing East Road, Nanchang City, Jiangxi Province 330029, People’s Republic of China Full list of author information is available at the end of the article
In addition, OSCC can be easily diagnosed as other oral lesions [5], leading to delayed treatment. Therefore, novel therapeutic targets and accurate diagnostic markers are urgently needed. The tumorigenesis and progression of OSCC are complicated and involve multiple genetic processes [6, 7]. The development of OSCC may provide new insights into its treatment and prevention. Long non-coding RNAs (lncRNAs, > 200 nt) are frequently dysregulated in cancer and may regulate cancer-related gene expression [8, 9]. Therefore, lncRNAs are promising targets for the development o
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