Micro and Nano Technologies in Bioanalysis Methods and Protocols
In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across th
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
METHODS
IN
MOLECULAR BIOLOGY™
Micro and Nano Technologies in Bioanalysis Methods and Protocols
Edited by
James Weifu Lee and Robert S. Foote Oak Ridge National Laboratory, Oak Ridge, TN, USA
Editors James Weifu Lee Oak Ridge National Laboratory Oak Ridge TN, USA
Robert S. Foote Oak Ridge National Laboratory Oak Ridge TN, USA
ISSN: 1064-3745 e-ISSN: 1940-6029 ISBN: 978-1-934115-40-4 e-ISBN: 978-1-59745-483-4 DOI: 10.1007/978-1-59745-483-4 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009929345 © Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is for-bidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with re-spect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
This book provides current information on the development of microfluidics, nanotechnologies, and physical science techniques for the separation, detection, manipulation, and analysis of biomolecules, and should be useful to a wide audience, including molecular and cell biologists, biochemists, microbiologists, geneticists, and medical researchers. Chapters cover a variety of topics and techniques ranging from lab-on-chip technologies and microfluidics-coupled mass spectrometry for separation and detection of biomolecules, including proteins and nucleic acids, to manipulating and probing biomolecules with nanopores, nanochannels, optical, and other physical means, with the possibility of isolation and analysis of individual biomolecules from a single cell, and to structural and functional analysis of biomolecules with liquid nuclear magnetic resonance, X-ray and neutron scattering techniques. The book presents emerging nanotechnologies including quantum dots and molecular fluorescence for imaging and tracking o
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