Microarray Data Analysis Methods and Applications
This volume covers a large area, from the description of methodologies for data analysis to the real application. Chapters focus on methodologies for preprocessing of microarray data, a survey of miRNA Data analysis, Cloud-based approaches, application of
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Pietro Hiram Guzzi Editor
Microarray Data Analysis Methods and Applications Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Microarray Data Analysis Methods and Applications Second Edition
Edited by
Pietro Hiram Guzzi Department of Surgical and Medical Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, Italy
Editor Pietro Hiram Guzzi Department of Surgical and Medical Sciences University “Magna Græcia” of Catanzaro Catanzaro, Italy
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3172-9 ISBN 978-1-4939-3173-6 (eBook) DOI 10.1007/978-1-4939-3173-6 Library of Congress Control Number: 2016932899 Springer New York Heidelberg Dordrecht London # Springer Science+Business Media New York 2007, 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The development of novel technological platforms in molecular biology has given a large input to research and in particular has caused a big development of bioinformatics to support storage, management, and analysis of a large amount of data about different aspects of the omic world. We here in particular focus on two main techniques for studying the activity of transcriptome, i.e., the set of molecules that play a role in the complex mechanism of protein synthesis. Such a study focuses on the role of mRNA, i.e., coding fragments of messenger RNA, and miRNA, i.e., small fragments of noncoding RNA. This study has been conducted through two main technological platforms: microarray and miRNA-microarray. More recently, the advent of next-generation sequencing techniques is gaining a prominent role. Despite this, classical micr
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