Protein Microarray for Disease Analysis Methods and Protocols
Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral im
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Protein Microarray for Disease Analysis Methods and Protocols Edited by
Catherine J. Wu Division of Hematologic Neoplasia, Department of Medical Oncology, Cancer Vaccine Center, Dana-Farber Cancer Institute, Boston, MA, USA
Editor Catherine J. Wu Division of Hematologic Neoplasia Department of Medical Oncology Cancer Vaccine Center Dana-Farber Cancer Institute Boston, MA 02115 USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-042-3 e-ISBN 978-1-61779-043-0 DOI 10.1007/978-1-61779-043-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011921931 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Protein microarrays are a rapidly growing segment of proteomics that enable highthroughput discovery-driven research through direct measurement of the molecular endpoints of various physiological and pathological states. The human genome has some 30,000 protein-coding genes, while the human proteome is estimated to have at least 90,000 proteins. By now, protein microarrays have been used for identifying protein– protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In this volume, we provide concise descriptions of the methodologies to fabricate microarrays for comprehensive analysis of proteins or the response to proteins that can be used to dissect human disease. These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes. Three general categories of array
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