Microbial Metabolic Engineering Methods and Protocols
Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. In Microbial Metabolic Engineering: Methods and Protocols expert researc
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Microbial Metabolic Engineering Methods and Protocols Edited by
Qiong Cheng DuPont Central Research and Development, Wilmington, DE, USA
Editor Qiong Cheng DuPont Central Research and Development Wilmington, DE, USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-482-7 e-ISBN 978-1-61779-483-4 DOI 10.1007/978-1-61779-483-4 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011943294 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Classic strain improvement is a long process that relies on screening of naturally occurring or chemically induced mutations for improved strain performance. Rapid technology advancement has accelerated strain improvement by opening the field of metabolic engineering. Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. The approaches may be as simple as manipulation of a single gene, and it could also be as complex as reconstruction of the cell’s networks. The objective of Microbial Metabolic Engineering is to provide an overview of strategies and techniques of metabolic engineering within the scope of microbial applications mainly focused on bacteria and yeasts. The first part of the book describes methods to engineer genes, pathways, or the whole genome in the production host. The complementary approaches of rational design and random screening to tap into natural diversity and engineered diversity are both illustrated. The second part of the book describes the use of modern biotechnology tools in microbial metabolic engineering. It includes use of genetic tools, omics tools, FACS analysis, and flux balance analysis for identification of targets of genes/ pathways for metabolic engineering. It also includes use of microfermentors and fermentation control techniques for rapid evaluation of engineered strains. The third part of the book describes several succes
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