Microbial Proteomics Methods and Protocols
This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractiona
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Dörte Becher Editor
Microbial Proteomics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Microbial Proteomics Methods and Protocols
Edited by
Dörte Becher Department of Microbial Proteomics, Institute for Microbiology, University Greifswald, Greifswald, Germany
Editor Do¨rte Becher Department of Microbial Proteomics Institute for Microbiology University Greifswald Greifswald, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8693-4 ISBN 978-1-4939-8695-8 (eBook) https://doi.org/10.1007/978-1-4939-8695-8 Library of Congress Control Number: 2018955428 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedication This book is dedicated to Michael Hecker, a pioneer in the field of microbial proteomics, in recognition of his pivotal scientific contributions
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Preface Considering the fact that microbes are by far the most abundant organisms on earth, there is no doubt that microbes and their activities are important to almost all processes on our planet. They affect all aspects of human life, as they are ubiquitously present in the environment and human microbiota, and contribute to human health and disease. Therefore, it is not surprising that microorganisms and microbiota are the focus of research to understand their physiology. The understanding of metabolic processes, stress adaptation, and survival strategies will lead to optimal conditio
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