Glyco-Engineering Methods and Protocols
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and
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Alexandra Castilho Editor
GlycoEngineering Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Glyco-Engineering Methods and Protocols
Edited by
Alexandra Castilho University of Natural Resources and Life Sciences, Vienna, Austria
Editor Alexandra Castilho University of Natural Resources and Life Sciences Vienna, Austria
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2759-3 ISBN 978-1-4939-2760-9 (eBook) DOI 10.1007/978-1-4939-2760-9 Library of Congress Control Number: 2015941740 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Glycosylation is a critical modification that confers and controls a range of properties to a protein. The diverse function of glycoproteins is a direct result of their structure, and the majority of the currently approved protein pharmaceutics rely on proper glycosylation to exhibit optimum efficacy. Glycosylation is considered to be one of the main causes for protein heterogeneity and is seen as a critical quality attribute by regulatory agencies. Specific glycosylation patterns obtained through glyco-engineering are expected to play a central role in future bio-manufacturing processes. The fact that glycoproteins, particularly therapeutic proteins, are in great demand have stimulated an extensive interest and search for new, better, and faster sources for recombinant protein production. However, depending on its source, the glycosylation pattern of a recombinant protein may vary greatly. Controlling glycosylation in vivo through modulation of
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