Microbial Toxins Methods and Protocols

Interest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines. In an effort to supplement the available reference texts on toxins, Microbial Toxins: Methods and Protocols includes protocols on mo

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Molecular Biology™

Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK



For other titles published in this series, go to www.springer.com/series/7651

Microbial Toxins Methods and Protocols Edited by

Otto Holst Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Parkallee 4a/c, 23845, Borstel, Germany

Editor Otto Holst Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften Parkallee 4a/c 23845, Borstel Germany [email protected]

ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-101-7 e-ISBN 978-1-61779-102-4 DOI 10.1007/978-1-61779-102-4 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011928553 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or ­dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, ­neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)

Preface In the year 2000, a first methods collection entitled Bacterial Toxins: Methods and Protocols, which contained 20 chapters on protein toxins and endotoxin from bacteria and cyanobacteria, was published. The idea was to support researchers of various scientific disciplines with detailed descriptions of state-of-the-art protocols and, since the book turned out to be quite successful, it is quite obvious that this aim could be achieved. However, it was also noticed that by focusing on bacterial toxins one significant area of toxin research was missing, namely, mold fungus toxins. Now, 10 years later, a second volume entitled Microbial Toxins: Methods and Protocols, which includes protocols on mold fungus toxins with some focus on aflatoxins, is presented. The interest of researchers across a broad spectrum of scientific disciplines in the field of microbial toxins is clearly unbroken. Since this field, as many others do, makes use of a large variety of biological, chemical, physical, and medical approaches, investigators dealing wi