Microtubule Dynamics Methods and Protocols
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynami
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Microtubule Dynamics Methods and Protocols
Edited by
Anne Straube Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, Coventry, UK
Editor Anne Straube Centre for Mechanochemical Cell Biology Warwick Medical School University of Warwick Coventry, UK [email protected]
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ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-251-9 e-ISBN 978-1-61779-252-6 DOI 10.1007/978-1-61779-252-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011931971 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Microtubules are at the heart of cellular self-organization and the target of the most widely used anticancer drugs. The dynamic nature of microtubules allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. They provide physical strength and, through dynamic interactions with subcellular structures, such as kinetochores, cell adhesion sites, or the actin cortex, orchestrate multiple cell biological processes, including chromosome segregation and directional cell migration. Modern methods make it possible to ask for mechanistic principles underlying microtubule dynamics regulation. The main purpose of this book is to provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. While it was impossible to cover all available techniques to study microtubule dynamics, I hope this book provides detailed protocols how to perform a broad range of well-established and newly emerging techniques, contributed by experts in the field. This book was put together with two groups of readers in mind: First, students and postdoctoral researchers starting work in a microtubule laboratory. Second, established researchers in the microtubule field who require a resource for established a
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