Mitochondria Practical Protocols
This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo.
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Dejana Mokranjac Fabiana Perocchi Editors
Mitochondria Practical Protocols Second Edition
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Mitochondria Practical Protocols Second Edition
Edited by
Dejana Mokranjac Biomedical Center-Physiological Chemistry, LMU Munich, Martinsried, Germany
Fabiana Perocchi Gene Center, LMU Munich and Institute for Diabetes and Obesity, Helmholtz Zentrum München, Munich, Germany
Editors Dejana Mokranjac Biomedical Center-Physiological Chemistry LMU Munich Martinsried, Germany
Fabiana Perocchi Gene Center, LMU Munich and Institute for Diabetes and Obesity Helmholtz Zentrum München Munich, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6822-0 ISBN 978-1-4939-6824-4 (eBook) DOI 10.1007/978-1-4939-6824-4 Library of Congress Control Number: 2017930957 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: mitochondria in mammalian HeLa cells. See Chapter 17 for details. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface It is very hard, if not impossible, to determine who first discovered mitochondria in the second half of the nineteenth century. Carl Benda, a German anatomist and pathologist, was the first, in 1898, to use the name mitochondrion (after Greek mitos, a thread, and chondros, a grain) to describe peculiar threads of granules he observed in the cytoplasm of cells after staining with crystal violet. For over a century, mitochondria have fascin
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