Plant Mitochondria Methods and Protocols
The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein, and enzymatic activities. Given that the ability to unc
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James Whelan Monika W. Murcha Editors
Plant Mitochondria Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M.Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Mitochondria Methods and Protocols
Edited by
James Whelan La Trobe University, Bundoora, VIC, Australia
Monika W. Murcha The University of Western Australia, Crawley, WA, Australia
Editors James Whelan La Trobe University Bundoora, VIC, Australia
Monika W. Murcha The University of Western Australia Crawley, WA, Australia
Additional material to this book can be downloaded from http://extras.springer.com. ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2638-1 ISBN 978-1-4939-2639-8 (eBook) DOI 10.1007/978-1-4939-2639-8 Library of Congress Control Number: 2015937422 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Foreword Highlights in Plant Mitochondrial Research As someone who started their mitochondrial research in the 1970s, it is interesting to see how far the field has progressed in terms of methodologies and experimental approach and how our knowledge of the way mitochondrial function affects the entire cell’s metabolism and gene networks has advanced. Table 1 illustrates the major milestones in this journey. The late 1950s and 1960s were preoccupied with determining the seat of oxidative phosphorylation and associated metabolism within cells, and once that was more or less accepted, the next two decades were devoted to investigating the mitochondrial genome structure, the composition of the electron transport chain, the basic carbon and nitrogen metabolism as
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