Molecular diversity of Egyptian cotton ( Gossypium barbadense L.) and its relation to varietal development
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J. Crop Sci. Biotech. 2012 (June) 15 (2) : 93 ~ 99 DOI No. 10.1007/s12892-011-0120-5 RESEARCH ARTICLE
Molecular Diversity of Egyptian Cotton (Gossypium barbadense L.) and its Relation to Varietal Development Kamal Fouad Abdellatif1*, Yehia Abdallah Khidr1, Yasser Mohammad El-Mansy2, Mohammad Mohammad El-Lawendey2, Yasser Abdelraouf Soliman2 Plant Biotech. Dept., GEBRI, Sadat City, Minoufiya Univ., Egypt Cotton Research Institute, Agricultural Research Center, Giza, Egypt
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Received: December 16, 2011 / Revised: February 15, 2012 / Accepted: March 13, 2012 Ⓒ Korean Society of Crop Science and Springer 2012
Abstract Twenty-eight Egyptian cotton (Gossypium barbadense L.) genotypes (varieties and hybrids) were used for analysis of genetic diversity using DNA based markers (ISSR, SSR, and EST) and to study varietal development of cotton. The ISSR markers gave the highest percentage of polymorphic bands as well as polymorphic information content compared with the other molecular markers (i.e. EST and SSR markers). Using clustering analysis, no general clustering according to the pedigree history of the genotypes was observed. Using principal coordinate analysis (PCOORDA), cotton genotypes were separated by the first three principal coordinates (PC1, PC2, and PC3) accounting for 11.5, 8.6, and 7.2% of the total genetic variance, respectively. The cotton genotypes were distributed into three parts based on the first PC, each part containing a group of varieties having a common ancestor. ‘Giza 12’ variety was the common ancestor for the varieties included in the first part and ‘Ashmouni’ variety was the common ancestor for the varieties included in the second part, while both ‘Sakha 3’ and ‘Sakha 4’ varieties were common ancestors for the varieties included in the third part. The results of the PCOORDA also showed better resolution of the genetic diversity than cluster analysis especially in the illustration of the varietal development of cotton. That means that principal coordinate analysis can be strongly used either alone or in combination with cluster analysis to discuss both genetic diversity and varietal development in the cotton genotypes. Key words: cluster analysis, Egyptian cotton, genetic diversity, molecular markers, PCOORDA
Introduction Cotton is the world’s most important fiber crop and the second most important oilseed crop. The primary product of the cotton plant has been the lint that covers the seeds within the seed pod, or boll. This lint has been utilized for thousands of years for clothing the people of ancient India, Asia, the Americas, and Africa. Cotton fabrics have been found in excavations at Mohenjo-Daro in India and in pre-Inca cultures in the Americas (Hutchinson et al. 1947). It is grown on every continent except Antarctica and in over 60 countries around the world. In many countries, cotton is one of the primary economic bases, providing employment and income for millions of people involved in its production, processing, and marketing (United Nations 2003). Kamal Fouad Abdellatif (
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