Molecular weight of polyethylenimine-dependent transfusion and selective antimicrobial activity of functional silver nan
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Molecular weight of polyethylenimine-dependent transfusion and selective antimicrobial activity of functional silver nanoparticles Atul Kumar Tiwari1, Munesh Kumar Gupta2, Govind Pandey3, Roger J. Narayan4,a), Prem C. Pandey1,a) 1
Department of Chemistry, Indian Institute of Technology, Varanasi, Uttar Pradesh 221005, India Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India 3 Department of Pediatrics, King George Medical University, Lucknow, Uttar Pradesh 226003, India 4 Department of Biomedical Engineering, North Carolina State University, North Carolina 27695, USA a) Address all correspondence to this authors. e-mail: [email protected] and [email protected] 2
Received: 30 April 2020; accepted: 29 June 2020
Synthetic cationic polymer-mediated synthesis of silver nanoparticles and selective antimicrobial activity of the same were demonstrated. Polyethyleneimine (PEI)-coated silver nanoparticles showed antimicrobial activity against Acinetobacter baumannii as a function of the polymeric molecular weight (MW) of PEI. Silver nanoparticles were coated with PEI of three different MWs: Ag-NP-1 with PEI exhibiting a MW of 750,000, Ag-NP-2 with PEI exhibiting a MW of 1300, and Ag-NP-3 with PEI exhibiting a MW of 60,000. These nanoparticles showed a particle size distribution of 4–20 nm. The nanoparticles exhibited potent antimicrobial activity against A. baumannii, with the minimum inhibitory concentration of Ag-NP-1, Ag-NP-2, and Ag-NP-3 on the order of 5, 10, and 5 μg/mL, respectively, and minimum bactericidal concentration of Ag-NP-1, Ag-NP-2, and Ag-NP-3 on the order of 10, 20, and 10 μg/mL, respectively. Fluorescence imaging of Ag-NPs revealed selective transfusion of Ag-NPs across the cell membrane as a function of the polymeric MW; differential interaction of the cytoplasmic proteins during antimicrobial activity was observed.
INTRODUCTION The technologies for introducing a cationic charge on inorganic materials (including nanoparticles) typically involve surface grafting with amine groups and coating with cationic polymers (e.g., polyethyleneimine (PEI), poly-amidoamine, and poly-lysine) through either covalent or electrostatic association [1,2,3]. PEIs are synthetic cationic polymers that compact DNA and siRNA into complexes, which are taken up in cells [3]. Since the cytotoxicity of PEI coating can interfere with the efficacy of the delivery system, it is possible that by selecting optimal polymeric lengths to control transfusion efficiency, one may simultaneously reduce or eliminate toxic effects of cationic polymer-coated nanoparticles. In turn, this directed us to investigate the synthesis and coating of silver nanoparticles (Ag-NPs) by PEI as a function of polymeric molecular weight (MW) [4,5]. We are interested in the interaction of PEI-coated Ag-NPs with multidrug-resistant (MDR) microbes [5,6,7,8]. Silver is
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considered to be a Lewis acid [9], which has the tendency to react with a Lewis b
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