AGL19 directly interacts with floral signal integrator AGL24 in flowering time control of Brassica juncea
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ORIGINAL ARTICLE
AGL19 directly interacts with floral signal integrator AGL24 in flowering time control of Brassica juncea Yuanda Wang1 · Wei Jiang1 · Yue Dong1 · Xiao Ma1 · Wenwen Zhou1 · Zhimin Wang1,2 · Yonghong Fan3 · Dayong Wei1,2 · Qinglin Tang1,2 Received: 16 February 2020 / Revised: 18 May 2020 / Accepted: 16 November 2020 © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2020
Abstract SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) and AGAMOUS-LIKE 24 (AGL24) are pivotal flowering signal integrators, which respectively, belong to TM3/SOC1 and STMADS11 clades of MIKC-type MADS-box family. AGAMOUS-LIKE 19 (AGL19), one member of TM3/SOC1 clade family, acts as an activator of flowering time. Previous studies have suggested that AGL19 together with AGL24 and SOC1 coordinately regulate flowering time. However, it remains unknown how AGL19 interacts with SOC1 and AGL24 in Brassica juncea. Here, an AGL19-like (BjuAGL19) was cloned from B. juncea, which was expressed in roots, stems, leaves, buds and flowers. Furthermore, both yeast one-hybrid and Dual-Glo Luciferase assays confirmed that BjuAGL19 protein could not target the promoters of BjuSOC1 and BjuAGL24. Interestingly, based on yeast two-hybrid and pull-down assays, BjuAGL19 protein directly interacted with BjuAGL24 protein, instead of BjuSOC1 protein. It is speculated that BjuAGL19 can bind to BjuAGL24 to form protein complex of BjuAGL19-BjuAGL24 and thus regulates flowering time in B. juncea. The results are very helpful for further elucidating the molecular mechanism of BjuAGL19 in flowering time control. Keywords Brassica juncea · Flowering time · AGL19 · Interaction
Introduction
Communicated by P. Wojtaszek. Yuanda Wang, Wei Jiang and Yue Dong contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11738-020-03163-4) contains supplementary material, which is available to authorized users. * Dayong Wei [email protected] * Qinglin Tang [email protected] 1
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
2
Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China
3
Chongqing Yudongnan Academy of Agricultural Sciences, Fuling 408000, China
Plant flowering time is controlled by hundreds of transcription factors, which are usually combined with each other through direct or indirect interactions to form a complex and delicate regulation network. Most of the regulatory factors belong to the MADS-box family (Smaczniak et al. 2012). Based on phylogenetic tree analysis, MADS-box family can be divided into type I and type II (Becker and Theissen 2003). AGAMOUS-LIKE 19 (AGL19) is a flowering regulator, which belongs to the type II MADS-box family (Kang et al. 2015). The expression of AGL19 is mainly detected not only in roots, but also in stems, leaves and flowers of Arabidopsis thaliana at a relative lower level (Schonrock et a
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