Inheritance and gene mapping of the white flower trait in Brassica juncea
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Inheritance and gene mapping of the white flower trait in Brassica juncea Xiangxiang Zhang & Rihui Li & Li Chen & Sailun Niu & Qun Li & Kai Xu & Jing Wen & Bin Yi & Chaozhi Ma & Jinxing Tu & Tingdong Fu & Jinxiong Shen
Received: 8 May 2017 / Accepted: 22 December 2017 / Published online: 30 January 2018 # Springer Science+Business Media B.V., part of Springer Nature 2018
Abstract Despite being a unique marker trait, white flower inheritance in Brassica juncea remains poorly understood at the gene level. In this study, we investigated a B. juncea landrace with white petal in China. The white petal phenotype possessed defective chromoplasts with less plastoglobuli than the yellow petal phenotype. Genetic analysis confirmed that two independent recessive genes (Bjpc1 and Bjpc2) controlled the white flower trait. We then mapped the BjPC1 gene in a BC4 population comprising 2295 individuals. We identified seven AFLP (amplified fragment length polymorphism) markers closely linked to the white flower gene. BLAST search revealed the sequence of AFLP fragments were highly homologous with the Scaffold000085 and Scaffold000031 sequences on the A02 chromosome in the Brassica rapa genome. Based on this sequence homology, we developed simple sequence repeat (SSR) primer pairs and identified 13 SSRs linked to the BjPC1 gene, including two that were cosegregated (SSR9 and SSR10). The two closest markers (SSR4 and SSR11) were respectively 0.9 and 0.4 cM on either side of BjPC1. BLAST analysis revealed that these marker sequences corresponded highly to A02 in Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-017-0771-0) contains supplementary material, which is available to authorized users. X. Zhang : R. Li : L. Chen : S. Niu : Q. Li : K. Xu : J. Wen : B. Yi : C. Ma : J. Tu : T. Fu : J. Shen (*) National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University, 430070 Wuhan, People’s Republic of China e-mail: [email protected]
B. juncea. They were mapped within a 33 kb genomic region on B. rapa A02 (corresponds to a 40 kb genomic region on B. juncea A02) that included three genes. Sequence BjuA008406, homologous to AtPES2 in Arabidopsis thaliana and Bra032956 in B. rapa, was the most likely candidate for BjPC1. These results should accelerate BjPC1 cloning and facilitate our understanding of the molecular mechanisms controlling B. juncea petal color. Keywords Brassica juncea . Flower color . Fine mapping . Molecular marker . Synteny
Introduction In nature, organisms use color cues to attract or repel others (Faegri and van der Pijl 1979). Among the most well-known of these cues is flower color, which acts as an appealing signal to pollinators (And and Baker 1983). Variation in flower color is mainly attributed to chemically distinct pigments (e.g., carotenoids, flavonoids, betalains, and chlorophylls) with carotenoids— responsible for yellows, oranges, and reds—being the most representative biocolor iso
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