Confirmation of Induced Tolerance to Alternaria Blight Disease in Indian Mustard ( Brassica juncea L.)
- PDF / 425,085 Bytes
- 14 Pages / 439.37 x 666.142 pts Page_size
- 70 Downloads / 189 Views
Confirmation of Induced Tolerance to Alternaria Blight Disease in Indian Mustard (Brassica juncea L.) P. D. Meena, et al. [full author details at the end of the article] Received: 18 April 2020 / Accepted: 22 June 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Indian mustard (Brassica juncea L.) is an important edible oilseed crop in India. Low productivity is the major concern which is adversely affected by biotic stresses. Alternaria blight (Alternaria brassicae) is one among major diseases that has no resistant cultivar until now. Keeping in view, an experiment was conducted for isolation of Alternaria blight– tolerant mutants in Indian mustard using gamma radiation and EMS mutagens during four consecutive years in Rabi (winter season). Furthermore, the morphologically and economically superior mutants of Brassica juncea were screened artificially at cotyledonary and adult stage against Alternaria blight. Tolerance to Alternaria blight is observed in DRMR-M163 (11.7%), DRMR-M-158 (13.1%), DRMR-M-174 (13.8%) and DRMR-M-177 (18.6%) with minimum conidia in infected cotyledons. Mutant DRMR-M-178 (19.8%) had the highest radical scavenging activity, while DRMR-M-162 (104.9 mg/g AAE), DRMR-M169 (96.9) and DRMR-M-161 (96.9) had higher antioxidant capacity that appears to act as defence to pathogen. DRMR-M-168 (8.4%), DRMR-M-173 (8.3), DRMR-M-171 (7.9), DRMR-M-165 (7.4), DRMR-M-175 (7.2) and DRMR-M-172 (6.9) had higher phenol content which may be responsive for resistance, although DRMR-M-161 (192.7 mg/g), DRMR-M-163 (187.7 mg/g), DRMR-M-164 (132.7 mg/g), DRMR-M-167 (149.3 mg/g), DRMR-M-173 (196.0 mg/g) and DRMR-M-178 (192.7 mg/g) mutants are found to contain low levels of total soluble sugar compared with susceptible Rohini (379.3). Based on biochemical parameter’s similarity, mutants are grouped in 4 major clusters. Cluster 4 contained significantly different mutant DRMR-M-172. Relative expression of mitogenactivated protein kinase 3 (MAPK3) gene was found highest in DRMR-M-177, DRMR-M174, DRMR-M-175, DRMR-M-178, DRMR-M-170, DRMR-M-176, DRMR-M-172 and DRMR-M-173 which resulted the better response to AB stress. Based on biochemical analysis, realtime PCR and cluster analysis, DRMR-M-172 mutant appears more tolerant to Alternaria. DRMR-M-178, DRMR-M-167 and DRMR-M-177 mutants seem tolerant and could be utilized for further breeding programme. Keywords Alternaria blight . Biochemical . Induced resistance . Indian mustard . Mutants
Introduction Indian mustard (Brassica juncea (L.) Czern & Coss.) is considered as one of the important oilseed crop planted in 6.23 mha area and produced a record of 9.34 mt with highest 1499 kg/
Applied Biochemistry and Biotechnology
ha productivity in India during 2018–2019. The increasing domestic demand of oil, seed and oil yield needs to be enhanced to fight the situation of overburden of edible oil import in India. Yield is one of the most important economic characters and is the product of multiplicative interaction of contributing characters. He
Data Loading...
