mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene
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mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene Wanjuan Li1, Huan Zhao1, Fuyang Ba2, Shaojuan Li1, Xiupeng Sun1, Dazuo Yang1*, Yibing Zhou1* 1 Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean
University, Dalian 116023, China 2 Marine and Fisheries Technology Center of Pan Shan Country, Panjin 124000, China
Received 6 June 2017; accepted 1 August 2017 © Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract
Rapid amplification of cDNA ends (RACE) and real-time polymerase chain reaction (RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene (BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity. Key words: Marphysa sanguinea, CYP4, benzo[a]pyrene, toxicity effect Citation: Li Wanjuan, Zhao Huan, Ba Fuyang , Li Shaojuan, Sun Xiupeng, Yang Dazuo, Zhou Yibing. 2019. mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene. Acta Oceanologica Sinica, 38(6): 46–53, doi: 10.1007/s13131-0191362-1
1 Introduction Polycyclic aromatic hydrocarbons (PAHs), which are composed of two or more aromatic nuclei, have been regarded as a primary pollution source in marine coastal environment (Martins et al., 2013). The oil spill in the Gulf of Mexico (2010) as well as in the Bohai Bay of China (2011), and ship wrecks such as the Prestige accident in 2002, the Erika accident in 1999 and the Volganeft-139 accident in 2010 have caused several millions of crude oil leaking into the sea, and its main content PAHs made serious impact on the marine environment in those areas. PAHs are persistent, toxic, and contain carcinogenic compounds that can attack DNA by forming PAH-DNA adducts and cause disorders in marine animals (Tsai et al., 2007). These compounds can also be transferred throughout the food chain and threaten higher levels of organisms, through biomagnification; as a result, various disorders, such as decreased fertility, obesity, and abnormal organ development, occured (Kraugerud et al., 2012; Lyche et al., 2011, 2013). Low-molecular-weight PAHs volatilize into the atmosphere, but high-molecular-weight PAHs subside in water and adsorb on the surface of sediment particles; the adsorbed PAHs then accum
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