Natural variation in glycine-rich region of Brassica oleracea cold shock domain protein 5 ( BoCSDP5 ) is associated with
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Genes & Genomics https://doi.org/10.1007/s13258-020-01010-x
RESEARCH ARTICLE
Natural variation in glycine-rich region of Brassica oleracea cold shock domain protein 5 (BoCSDP5) is associated with low temperature tolerance Hayoung Song1 · HyeRan Kim2 · Byung‑Ho Hwang3 · Hankuil Yi1 · Yoonkang Hur1 Received: 30 July 2020 / Accepted: 7 October 2020 © The Genetics Society of Korea 2020
Abstract Background Low temperature (LT) or cold stress is a major environmental stress that seriously affects plant growth and development, limiting crop productivity. Cold shock domain proteins (CSDPs), which are present in most living organism, are involved in RNA metabolisms influencing abiotic stress tolerance. Objective The aims of this study are to identify target gene for LT-tolerance, like CSDPs, characterize genetics, and develop molecular marker distinguishing LT-tolerance in cabbage (Brassica oleracea var. capitata). Methods Semi-quantitative RT-PCR or qRT-PCR was used in gene expression study. LT-tolerance was determined by electrolyte leakage and PCR with allelic specific primers. Results Allelic variation was found in BoCSDP5 coding sequence (CDs) between LT-tolerant (BN106 and BN553) and -susceptible inbred lines (BN107 and BN554). LT-tolerant inbred lines contained variant type of BoCSDP5 (named as BoCSDP5v) which encodes extra CCHC zinc finger domain at C-terminus. Association of LT-tolerance with BoCSDP5v was confirmed by electrolyte leakage and segregation using genetic population derived from BN553 and BN554 cross. Allelic variation in BoCSDP5 gene does not influence the rate of gene expression, but produces different proteins with different number of CCHC zinc finger domains. LT-tolerance marker designed on the basis of polymorphism between BoCSDP5 and BoCSDP5v was confirmed with samples used in previous B. oleracea CIRCADIAN CLOCK ASSOCIATED 1 (BoCCA1) marker validation. Conclusions LT-tolerant allele (BoCSDP5v) is dominant and independent of CBF pathway, and sufficient to generate molecular markers to identify LT-tolerant cabbage when it is used in combination with another marker, like BoCCA1-derived one. Production and analysis of overexpressing plants of BoCSDP1, BoCSDP3, BoCSDP5 and BoCSDP5v will be required for elucidating the function of CCHC zinc finger domains in LT-tolerance. Keywords Cold shock domain (CSD) · Allele variation · LT-tolerance · Cabbage · Molecular marker
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13258-020-01010-x) contains supplementary material, which is available to authorized users. 1
* Yoonkang Hur [email protected]
Department of Biological Sciences, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea
2
Hayoung Song [email protected]
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea
3
Biotechnology and Breeding Institute of Asia Seed Co., Icheon‑si, Gyeonggi‑do 17414, Republic of Korea
* Hank
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