New record of a monopartite begomovirus and papaya leaf curl betasatellite infecting Mirabilis jalapa in Himachal Prades
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New record of a monopartite begomovirus and papaya leaf curl betasatellite infecting Mirabilis jalapa in Himachal Pradesh, India Aditya Kulshreshtha1,2 · Poonam Roshan1,2 · Amit Kumar Kesharwani2 · Vipin Hallan1,2 Received: 12 March 2020 / Revised: 13 July 2020 / Accepted: 5 October 2020 © Indian Phytopathological Society 2020
Abstract Mirabilis jalapa is an important plant species for its ornamental and medicinal values. In September 2013, M. jalapa leaf samples showing vein yellowing symptoms were collected from Kangra district of Himachal Pradesh and examined for begomovirus infection. PCR analysis using DNA-A and betasatellite specific primers gave desired amplification in all infected samples. However, no PCR amplification was obtained for DNA-B that suggested monopartite nature of the infecting virus. Sequence analysis showed that the partial begomovirus sequence was most similar (92%) to mirabilis leaf curl virus (MLCV). The full-length betasatellite (1374 bp) showed highest identity (99%) to papaya leaf curl betasatellite (PaLCuB), reported from Valeriana jatamansi. Phylogenetic analysis clustered begomovirus sequence with MLCV whereas the betasatellite sequence clustered with various isolates of PaLCuB reported from India and Pakistan. This is the first report of a monopartite begomovirus and papaya leaf curl betasatellite on a new host, M. jalapa. Keywords Begomovirus · Mirabilis jalapa · Papaya leaf curl betasatellite · Vein yellowing Mirabilis jalapa, the “4” O clock plant” belongs to Nyctaginaceae family, and produce flowers in a variety of colours. This plant has therapeutic applications and is used in many traditional medicines for the treatment of various disease (Aoki et al. 2008; Rozina 2016). Till now, only two begomoviruses, mirabilis leaf curl virus (MLCV) and chilli leaf curl India virus (ChiLCINV) were found to infect M. jalapa (Jaidi et al. 2017; Kulshreshtha et al. 2017). In September 2013, we observed vein yellowing, stunting and upward leaf curling on M. jalapa (Fig. 1) from Kangra district of Himachal Pradesh (32.0873° N, 76.2561° E; 760 m altitude), India. Based on disease symptoms and presence of whiteflies on the leaves, begomovirus infection was suspected. Plant leaf samples were collected and total DNA was extracted from 6 symptomatic and one non-symptomatic plant leaves as described (Rouhibakhsh et al. 2008). The viral genomic content was enriched via rolling circle * Vipin Hallan [email protected] 1
Academy of Scientific and Innovative Research (AcSIR), CSIR-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Palampur, Himachal Pradesh 176061, India
Plant Virus Lab, Division biotechnology, CSIR-IHBT, Palampur, Himachal Pradesh 176061, India
2
amplification (RCA) and then used as a template in a PCR reaction using conserved DNA-A; AV494: 5′-GCCY ATR TA YAGRAAGCCMAG-3′ and AC1048: 5′-GGRTTDGAR GCATGHGTACATG-3′ (Wyatt and Brown 1996), DNAB; PBL1v2040: 5′-GCCTCTG CAG CARTGRTCK ATC TTCATACA-3’ and PCRc1 5’-CTAGCTGCAGCATAT TTACRA
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