Occurrence and molecular variability of apple-associated Luteovirus isolates from apple in China based on coat protein g

  • PDF / 367,296 Bytes
  • 6 Pages / 547.087 x 737.008 pts Page_size
  • 116 Downloads / 232 Views

DOWNLOAD

REPORT


Occurrence and molecular variability of apple-associated Luteovirus isolates from apple in China based on coat protein genes Zhengnan Li & Mingxiu Ju & Lei Zhang & Mengji Cao & Pingping Sun

Received: 14 September 2019 / Revised: 8 September 2020 / Accepted: 22 October 2020 # Koninklijke Nederlandse Planteziektenkundige Vereniging 2020

Abstract Apple-associated Luteovirus (AaLV) was recently found in apple trees in China. How commonly AaLV occurs in apple and how much genetic diversity is present among samples from various locations and apple cultivars are not reported but are investigated here. In this study, 162 samples from 13 locations, covering 13 apple cultivars of Malus pumila and one wild apple of Malus sieversii in China were collected, their coat protein (CP) genes were amplified by reverse transcription-polymerase chain reaction and the variability of the CP genes was analysed. The occurrence ratio of AaLV was 37.04% in apple trees in China, the AaLV isolates showed little variation on their CP genes, the geographic location or host cultivars had no consistent effect on the occurrence, variation and phylogenetic characters of AaLV. The results of this study will provide information on further study of the molecular characteristics and epidemiology of AaLV.

Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007 /s10658-020-02149-9. Z. Li : M. Ju : L. Zhang : P. Sun (*) College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Zhaowuda Road No. 306, SaihanInner MongoliaHohhot 010018, China e-mail: [email protected] M. Cao (*) Citrus Research Institute, Southwest University, Tiansheng Road No. 2, Beifu, Chongqing 400715, China e-mail: [email protected]

Keywords Apple-associated Luteovirus . Apple . Occurrence . Molecular variation . Coat protein

Apple is an economically important fruit crop. It is mainly produced by vegetative propagation, which can result in an accumulation of a high titre of virus pathogens in apple trees through multiple manipulations, leading to significant damage and yield loss. According to previous reports, more than 10 species of viruses and viroids can infect apple trees, causing many diseases that reduce fruit quality and yield (Barba et al. 2015; Noda et al. 2017; Shen et al. 2018; Liu et al. 2018; Rott et al. 2018). Among them, apple stem pitting virus, apple stem grooving virus, and apple chlorotic leaf spot virus, each belonging to the family Betaflexiviridae, are the most commonly reported viruses in apple. Appleassociated luteovirus (AaLV) and apple luteovirus 1 (ALV1), belonging to the genus Luteovirus, are the two newest reported viruses. Luteoviruses infect more than 45 plant species spread over more than 40 countries (Muhammad et al. 2018). At present, six members of the genus are reported in woody plants, including rose spring dwarf-associated virus (RSDaV) (Salem et al. 2008), nectarine stem pitting-associated virus (NSPaV) (Bag et al. 2015), cherry-associated luteovirus (C