Occurrence of Alaria alata in wild boars ( Sus scrofa ) in Poland and detection of genetic variability between isolates
- PDF / 747,688 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 14 Downloads / 189 Views
HELMINTHOLOGY - ORIGINAL PAPER
Occurrence of Alaria alata in wild boars (Sus scrofa) in Poland and detection of genetic variability between isolates Ewa Bilska-Zając 1 & Gianluca Marucci 2 & Anna Piróg-Komorowska 3 & Małgorzata Cichocka 3 & Mirosław Różycki 1 & Jacek Karamon 1 & Jacek Sroka 1 & Aneta Bełcik 1 & Iwona Mizak 1 & Tomasz Cencek 1 Received: 18 March 2020 / Accepted: 29 September 2020 # The Author(s) 2020
Abstract Alaria alata is a trematode included among several emerging zoonotic parasites. The mesocercarial larval stage of A. alata named Distomum musculorum suis (DMS) may potentially be infective for humans. In the past, DMS was often observed in wild boar meat during the official Trichinella inspection by artificial digestion before a more specific and effective detection method, the A. alata mesocercariae migration technique (AMT), was introduced. In the present study, the AMT method was used to screen 3589 tissue samples collected from wild boars hunted in Poland during the 2015–2019 period. The survey mainly focused on the southern part of Poland with the majority of samples coming from Małopolskie, Świętokrzyskie, and Dolnoślaskie provinces; samples from ten additional provinces were also included. The total prevalence was 4.2% with mean abundance of 4.7 DMS. Occurrence was dependent upon environmental conditions (i.e., wetland habitats and water reservoirs) rather than on sex of the host or season in which they were hunted. The recovered trematodes were identified as Alaria spp. according to their morphological features. Molecular analysis of 18S rDNA and COI genes confirmed the species identification to be A. alata and documented genetic variability among the isolates. Keywords Alaria alata . DMS . AMT . Wild boars . Poland
Introduction Alaria alata (Diplostomidae, Trematoda), is a trematode discovered by Goeze in 1782. The mesocercarial stage of A. alata named Distomum musculorum suis (DMS) is the etiologic agent of a zoonosis known as alariosis. Currently, A. alata is considered an emerging zoonotic parasite. The life cycle of this trematode includes two intermediate hosts and various Section Editor: Christoph G. Grevelding Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00436-020-06914-x) contains supplementary material, which is available to authorized users. * Ewa Bilska-Zając [email protected] 1
National Veterinary Research Institute in Puławy, Al. Partyzantów 57, Puławy, Poland
2
Istituto Superiore di Sanità, Viale Regina Elena, 299 Rome, Italy
3
Veterinary Hygiene Laboratory, ul. Brodowicza 13a, Krakow, Poland
paratenic and definitive hosts. The first intermediate host is a freshwater snail (e.g., Helisoma, Planorbis spp.), which become infected by miracidia, the A. alata hatchling stage. The miracidia develop into sporocysts that produce cercaria, a fastmoving larval stage that emerges from its snail host and penetrates a tadpole and develops into a non-reproductive form, the mesocercaria. These mesocercariae can infect p
Data Loading...
