Oleuropein prevents ethanol-induced gastric ulcers via elevation of antioxidant enzyme activities in rats
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ORIGINAL PAPER
Oleuropein prevents ethanol-induced gastric ulcers via elevation of antioxidant enzyme activities in rats Masoud Alirezaei & Omid Dezfoulian & Shima Neamati & Marzyeh Rashidipour & Nader Tanideh & Arash Kheradmand
Received: 17 January 2012 / Accepted: 3 May 2012 / Published online: 13 May 2012 # University of Navarra 2012
Abstract Purified oleuropein from olive leaf extract has been shown to have antioxidant effects in our recent studies. Thus, the aim of this study was to assess the antioxidant abilities of oleuropein in comparison with ranitidine in ethanol-induced gastric damages via evaluation of ulcer index inhibition, antioxidant enzyme activities, and lipid peroxidation level. Fifty-six adult male Sprague–Dawley rats were divided into seven equal groups as follows: control group, ethanol group
(absolute ethanol 1 ml/rat), oleuropein group (12 mg/kg), and oleuropein (6, 12, and 18 mg/kg) plus ethanol groups, as well as ranitidine (50 mg/kg) plus ethanol group. Pretreatment with oleuropein (12 and 18 mg/kg) significantly increased the ulcer index inhibition (percent), in comparison with oleuropein (6 mg/kg). Glutathione peroxidase (GPx) activity was significantly lower in the ethanol group when compared with the other groups whereas, treatment of rats with oleuropein (12 mg/kg)
Electronic supplementary material The online version of this article (doi:10.1007/s13105-012-0177-8) contains supplementary material, which is available to authorized users. M. Alirezaei (*) Division of Biochemistry, School of Veterinary Medicine, Lorestan University, P.O. Box: 465, Khorram Abad, Iran e-mail: [email protected] M. Alirezaei Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorram Abad, Iran O. Dezfoulian Department of Pathobiology, School of Veterinary Medicine, Lorestan University, P.O. Box: 465, Khorram Abad, Iran S. Neamati Brojerd Branch, Young Researchers Club, Islamic Azad university, Lorestan, Iran
M. Rashidipour Khorram Abad Branch, Young Researchers Club, Islamic Azad University, Lorestan, Iran
N. Tanideh Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
A. Kheradmand Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, P.O. Box: 465, Khorram Abad, Iran
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significantly increased glutathione content in gastric tissue when compared with the other groups, and lipid peroxidation was significantly reduced in the oleuropein(12 and 18 mg/kg) and ranitidine-treated animals. Superoxide dismutase (SOD) and catalase (CAT) activities were both much higher in oleuropein-treated rats than the ethanol group, and although there was a moderate increase in SOD and CAT activities in ranitidine-treated rats, the differences were not significant. These findings suggest that oleuropein has beneficial antioxidant properties against ethanol-induced gastric damages in the rat. Therefore, it seems that a combination regimen including both antioxidant and antisecretory drugs may be beneficia
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