Outer inflammatory protein of Helicobacter pylori impacts IL-8 expression, adherence, cell apoptosis and cell cycle of g
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ORIGINAL INVESTIGATION
Outer inflammatory protein of Helicobacter pylori impacts IL‑8 expression, adherence, cell apoptosis and cell cycle of gastric cells independent of its copy number Qiaoyun Zhao1,2,3 · Wenzhu Yin4 · Rulin Zhao1,2,3 · Youhua Wang1,3 · Conghua Song1,2,3 · Huan Wang1,2,3 · Jianfang Rong1,2,3 · Fangfei Wang1,2,3 · Yong Xie1,2,3 Received: 9 May 2020 / Accepted: 23 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Outer inflammatory protein (OipA) is an important virulence factor of Helicobacter pylori (H. pylori), but the correlation between oipA copy number and its virulence remains unknown. The study was designed to investigate whether the duplicate oipA gene loci showed more virulent than one oipA gene in vitro. H. pylori strain CCS9803 (China Chongqing Strain 9803) that carries duplicate oipA loci was used to construct one or two oipA knockout mutant strain, which was further verified by qPCR and western blot. Gastric epithelial cells AGS and GES-1 were infected with wild-type (WT) or oipA mutants for 6 or 24 h. The expression levels of IL-8, bacterial adhesion, cell apoptosis and cell cycle were performed to analyze the function of oipA. The WT and oipA mutant strains induce significantly higher mRNA and protein levels of IL-8 than the uninfected group (P
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