A SBP-Box Gene VpSBP5 from Chinese Wild Vitis Species Responds to Erysiphe necator and Defense Signaling Molecules
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ORIGINAL PAPER
A SBP-Box Gene VpSBP5 from Chinese Wild Vitis Species Responds to Erysiphe necator and Defense Signaling Molecules Hongmin Hou & Qin Yan & Xiping Wang & Hui Xu
# Springer Science+Business Media New York 2013
Abstract A novel SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box gene, designated as VpSBP5, was isolated from Chinese wild Vitis pseudoreticulata clone “Baihe-35-1” infected by Erysiphe necator under natural field conditions. The full-length cDNA sequence of VpSBP5 comprised 3,811 bp and encoded a polypeptide of 1,030 amino acids which contained a highly conserved SBP domain bearing two zinc-binding sites of the C2HCH type and a nuclear localization signal. The deduced amino acid sequence of VpSBP5 was identical to that of VvSBP5 and grouped into the same clade (group 1) with AtSPL1, AtSPL12, AtSPL14, VvSBP7, and VvSBP17. We further confirmed that VpSBP5 gene is indeed targeted to the nucleus and possesses transcriptional activation activity. The expression of VpSBP5 as determined by reverse transcription PCR, was induced by E. necator in the E. necatorresistant V. pseudoreticulata clone Baihe-35-1 and the susceptible clone “Hunan-1,” and exhibited a quicker response in Baihe-35-1. Moreover, the expression of VpSBP5 was induced by salicylic acid (SA) and methyl jasmonate (MeJA) in Baihe-35-1. Together, our results indicate that VpSBP5 is likely to participate in the regulation of the resistance to E. necator by inducing SA and MeJA molecular signals in grape, and the degree of disease resistance of Electronic supplementary material The online version of this article (doi:10.1007/s11105-013-0591-2) contains supplementary material, which is available to authorized users. H. Hou : Q. Yan : X. Wang : H. Xu State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100 Shaanxi, China H. Hou : Q. Yan : X. Wang (*) : H. Xu Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling 712100 Shaanxi, China e-mail: [email protected]
the grapevine genotypes may correlate with the time of the peak appearing. Keywords Vitis pseudoreticulata . VpSBP5 . Erysiphe necator . Salicylic acid . Methyl jasmonate . Ethephon
Introduction Transcription factors are essential for regulating and controlling gene expression at the level of mRNA transcription by binding to a specific DNA site. They are capable of activating or repressing transcription of multiple target genes (Riechmann et al. 2000; Xiong et al. 2005). Many families of transcription factors enable plants to appropriately regulate growth, differentiation, and metabolism and to respond to endogenous and environmental cues by regulating the expression of specific downstream genes. A SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box gene family belongs to the type of plant-specific zinc finger protein genes, which encode putative plantspecific transcription factors. They all contain a highly conserved DNA-bindi
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