A celery transcriptional repressor AgERF8 negatively modulates abscisic acid and salt tolerance
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ORIGINAL ARTICLE
A celery transcriptional repressor AgERF8 negatively modulates abscisic acid and salt tolerance Jie‑Xia Liu1 · Bei Wu1 · Kai Feng1 · Meng‑Yao Li1 · Ao‑Qi Duan1 · Di Shen1 · Lian Yin1 · Zhi‑Sheng Xu1 · Ai‑Sheng Xiong1 Received: 3 February 2020 / Accepted: 12 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Ethylene response factors (ERFs) widely exist in plants and have been reported to be an important regulator of plant abiotic stress. Celery, a common economic vegetable of Apiaceae, contains lots of ERF transcription factors (TFs) with various functions. AP2/ERF TFs play positive or negative roles in plant growth and stress response. Here, AgERF8, a gene encoding EAR-type AP2/ERF TF, was identified. The AgERF8 mRNA accumulated in response to both abscisic acid (ABA) signaling and salt treatment. AgERF8 was proving to be a nucleus-located protein and could bind to GCC-box. The overexpression of AgERF8 in Arabidopsis repressed the transcription of downstream genes, AtBGL and AtBCH. Arabidopsis overexpressing AgERF8 gene showed inhibited root growth under ABA and NaCl treatments. AgERF8 transgenic lines showed low tolerance to ABA and salt stress than wild-type plants. Low increment in SOD and POD activities, increased accumulation of MDA, and significantly decreased plant fresh weights and chlorophyll levels were detected in AgERF8 hosting lines after treated with ABA and NaCl. Furthermore, the overexpression of AgERF8 also inhibited the levels of ascorbic acid and antioxidantrelated genes (AtCAT1, AtSOD1, AtPOD, AtSOS1, AtAPX1, and AtP5CS1) expression in transgenic Arabidopsis. This finding indicated that AgERF8 negatively affected the resistance of transgenic Arabidopsis to ABA and salt stress through regulating downstream genes expression and relevant physiological changes. It will provide a potential sight to further understand the functions of ERF TFs in celery. Keywords Celery · AgERF8 · Abscisic acid · Salt · Negative regulation · Tolerance
Introduction Plants may encounter various stresses throughout the lifecycle, such as high–low temperature, high salinity, drought, pests, and diseases. High salinity is one of the important environmental factors limiting agricultural production. Jie-Xia Liu, Bei Wu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00438-020-01738-x) contains supplementary material, which is available to authorized users. * Ai‑Sheng Xiong [email protected] 1
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China
Abscisic acid (ABA) is a major phytohormone that involved in the regulation on root growth and a key mediator in triggering plant responses to most of the abiotic stresses (Cutler et al. 2010; Sah et al. 20
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