Overexpression of the ThTPS gene enhanced salt and osmotic stress tolerance in Tamarix hispida
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ORIGINAL PAPER
Overexpression of the ThTPS gene enhanced salt and osmotic stress tolerance in Tamarix hispida Peilong Wang1 · Xiaojin Lei1 · Jiaxin Lü1 · Caiqiu Gao1
Received: 26 February 2020 / Accepted: 15 May 2020 © Northeast Forestry University 2020
Abstract Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses. Trehalose-6-phosphate synthase (TPS) plays important roles in trehalose metabolism and signaling. In this study, the full-length cDNA of ThTPS was cloned from Tamarix hispida Willd. A phylogenetic tree including ThTPS and 11 AtTPS genes from Arabidopsis indicated that the ThTPS protein had a close evolutionary relationship with AtTPS7. However, the function of AtTPS7 has not been determined. To analyze the abiotic stress tolerance function of ThTPS, the expression of ThTPS in T. hispida under salt and drought stress and JA, ABA and GA3 hormone stimulation was monitored by qRT-PCR. The results show that ThTPS expression was clearly induced by all five of these treatments at one or more times, and salt stress caused particularly strong induction of ThTPS in the roots of T. hispida. The ThTPS gene was transiently overexpressed in T. hispida. Both physiological indexes and staining results showed that ThTPS gene overexpression increased salt and osmotic Project funding: This work was supported by the Province in Heilongjiang Outstanding Youth Science Fund (JC2017004), the National Natural Science Foundation of China (No. 31370676) and Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team). The online version is available at http://www.springerlink.com. Corresponding editor: Yu Lei. * Caiqiu Gao [email protected] 1
State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin 150040, People’s Republic of China
stress tolerance in T. hispida. Overall, the ThTPS gene can respond to abiotic stresses such as salt and drought, and its overexpression can significantly improve salt and osmotic tolerance. These findings establish a foundation to better understand the responses of TPS genes to abiotic stress in plants. Keywords Trehalose-6-phosphate synthase (TPS) · Tamarix hispida · Salt tolerance · Osmotic resistance
Introduction Trehalose is a non-reducing disaccharide composed of two glucose molecules linked by α,α 1–1 glycosidic bonds (Elbein et al. 2003; Bansal et al. 2013). It was first discovered in bacteria by Wiggers in 1832, and the French chemist Berthelot subsequently discovered it in a sweet substance secreted by weevils in the Asia Minor Desert and named it trehalose (Drennan et al. 1999). Trehalose is widely found in various organisms such as bacteria, yeasts, molds, edible fungi, insects, lower plants such as algae and mosses, as well as some higher plants (Drennan et al. 1999; Goddijn and Dun 2006; Satoh-Nagasawa et al. 2006; Schluepmann et al. 2012; Jin et al. 2018; Alicandri et al. 2020). Trehalose ca
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