Required characteristics of Paenibacillus polymyxa JB-0501 as potential probiotic
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ORIGINAL PAPER
Required characteristics of Paenibacillus polymyxa JB‑0501 as potential probiotic Karim Naghmouchi · John Baah · Benoit Cudennec · Djamel Drider
Received: 5 March 2013 / Revised: 9 April 2013 / Accepted: 22 May 2013 / Published online: 12 June 2013 © Springer-Verlag Berlin Heidelberg 2013
Abstract The ability of Paenibacillus polymyxa to inhibit the growth of Escherichia coli generic ATCC 25922 (Escherichia coli ATCC 25922) and to adhere to monolayers of the enterocyte-like human cell line Caco-2 was evaluated. P. polymyxa JB-0501 (P. polymyxa JB-0501), found in a livestock feed probiotic supplement, was compared to P. polymyxa reference strain ATCC 43685 and ATCC 7070 (P. polymyxa ATCC) in terms of carbohydrate utilization and resistance to lysozyme, acid, bile salts, and hydrogen peroxide. JB-0501 grew at pH 4.5 and at H2O2 concentrations less than 7.3 μg/ml and presented a higher affinity to hexadecane and decane. Bile salts at 0.2 % inhibited the growth of all three strains. P. polymyxa JB-0501 and P. polymyxa ATCC 43865 adhered to Caco-2 cell monolayers. The percentage of cells that adhered ranged from about 0.35 to 6.5 % and was partially proportional to the number applied. Contact time (from 15 min to 1 h) had little impact on adhesion. P. polymyxa JB-0501 inhibited the growth of E. coli ATCC 25922, as proven by the diffusion
Communicated by Erko Stackebrandt. K. Naghmouchi (*) · J. Baah Lethbridge Research Center, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada e-mail: [email protected] K. Naghmouchi Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, El Manar, Tunis, Tunisia B. Cudennec · D. Drider (*) Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES‑EA 1026, Polytech’Lille/IUTA, Université Lille Nord de France, Avenue Paul Langevin, 59655 Villeneuve d’Ascq Cedex, France e-mail: djamel.drider@univ‑lille1.fr
tests in agar. Taken together, these results suggested that P. polymyxa JB-0501 has the potential probiotic properties to justify its consideration as a livestock feed supplement. Keywords Paenibacillus polymyxa JB-0501 · Escherichia coli · Caco-2 cells · Adhesion · Pathogenic bacteria · Probiotic properties
Introduction Paenibacillus polymyxa, formerly known as Bacillus polymyxa (Ash et al. 1993), constitutes part of microbiota from soil, rhizospheres, water, diseased insect larvae, and foods (Choi et al. 2009). These species have been reported as a potential biological control agent because of its capacity to produce a cyclic lipopeptide antibiotic, which is active against a wide variety of microbes such as Pseudomonas fluorescens C7R12, Streptomyces argentelous ATCC 1109, and Fusarium acuminatum DSM 62148 (Selim et al. 2005). Paenibacillus polymyxa JB-0501 (P. polymyxa JB-0501) was isolated from a microbial-based livestock feed supplement and was shown to be active against Gram-negative bacteria such as Escherichia coli RR1, Pseudomonas fluorescens R73, Pantoea agglomerans BC1, Butyri
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