Phylogeny, recombination, and invasiveness of group B Streptococcus revealed by genomic comparisons of its global strain
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ORIGINAL ARTICLE
Phylogeny, recombination, and invasiveness of group B Streptococcus revealed by genomic comparisons of its global strains Enze Lin 1,2,3 & Shengmei Zou 1,2,3 & Yue Wang 4 & Chien-Chung Lee 5,6 & Cheng-Hsun Chiu 5,6 & Ye Feng 1,2,3 Received: 14 April 2020 / Accepted: 9 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Capsular polysaccharide (CPS) genes and pilus islands encode important virulence factors for group B Streptococcus (GBS) genomes. This study aims to detect phylogenetic inconsistency in CPS genes and pilus islands in GBSs and to explore its relationship with invasiveness. A total of 1016 GBS genomes were downloaded from the NCBI public database. The multilocus sequence typing (MLST) and Bayesian analysis of Population Structure (BAPS) analyses were both conducted for phylogeny construction. Serotyping and pilus typing were determined in silico using the genomic sequences. The CPS and pilus typing results were generally consistent with MLST and BAPS clustering. GBS isolates of serotype II and of the PI-1 + PI-2b and PI-2a types were more prone to phylogenetic inconsistency than the others. Isolates of serotype Ib and of PI-1 + PI-2a were more likely to appear as colonizing strains, whereas PI-2b was more likely to appear in invasive strains. For serotype V, phylogenetic inconsistency occurred more commonly in colonizing isolates, while for serotype III, the opposite occurred. The present study profiles for the first time the phylogenetic inconsistency of CPS genes and pilus islands in global GBS isolates, which is helpful for infection control and the development of new vaccines for the prevention of GBS occurrence. Keywords Group B Streptococcus . Bayesian clustering . Multi-locus sequence typing . Pilus island . CPS type . Recombination . Invasiveness
Introduction Group B Streptococcus (GBS, Streptococcus agalactiae) was initially described as the cause of bovine mastitis [1]. In humans, GBS usually occurs in the genitourinary or gastrointestinal tract of adult males and females and is not pathogenic.
In the birthing process, however, GBS from the genital tract of pregnant women can infect the lower gastrointestinal and upper respiratory tracts of newborns [2], resulting in neonatal sepsis and meningitis. Even with the appropriate antibiotic treatment, the mortality rates of late-onset GBS infection (age 7–90 days [3]) are high [4].
Enze Lin, Shengmei Zou and Yue Wang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10096-020-04067-4) contains supplementary material, which is available to authorized users. * Cheng-Hsun Chiu [email protected]
3
Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, People’s Republic of China
* Ye Feng [email protected]
4
Women’s Hospital, Zhejiang University School of Medicine, Hangzhou Zhejiang, People’s Republic of China
5
Molecular Infectious Disease Research Center, Chang Gung Memo
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