Physico Chemical Characterization of Nanofibrous Poly( E -Caprolactone) Electrospun Templates for Cell Adhesion
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Physico Chemical Characterization of Nanofibrous Poly(Ǽ-Caprolactone) Electrospun Templates for Cell Adhesion Karla A. Gaspar-Ovalle, 1 Juan V. Cauich-Rodriguez2, Armando Encinas3 1) 2) 3)
Facultad de Estomatología, Universidad Autónoma de San Luis Potosí A. C., Av. Dr. Manuel Nava No. 2, Zona Universitaria, 78290 San Luis Potosí, SLP, México. Unidad de Materiales, Centro de Investigación Científica de Yucatán A.C., Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, 97245. Mérida, Yucatán, México. División de Materiales Avanzados, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José 2055, 78216 San Luis Potosí, SLP, México.
ABSTRACT Nanofibrous mats of poly İ-caprolactone (PCL) were fabricated by electrospinning. The nanofiber structures were investigated and characterized by scanning electron microscope, differential scanning calorimetry, thermogravimetric analysis, dynamic mechanical analysis, static water-contact-angle analysis and mechanical properties. The results showed that the nanofibrous PCL is an ideal biopolymer for cell adhesion, owing to its biocompatibility, biodegradability, structural stability and mechanical properties. Differential scanning calorimetry results showed that the fibrous structure of PCL does not alter its crystallinity. Studies of the mechanical properties, wettability and degradability showed that the structure of the electrospun PCL improved the tensile modulus, tensile strength, wettability and biodegradability of the nanotemplates. To evaluate the nanofibrous structure of PCL on cell adhesion, osteoblasts cells were seeded on these templates. The results showed that both adhesion and proliferation of the cells is viable on these electrospun PCL membranes. Thus electrospinning is a relatively inexpensive and scalable manufacturing technique for submicron to nanometer diameter fibers, which can be of interest in the commodity industry.
INTRODUCTION Polycaprolactone (PCL) is a relatively cheap biodegradable polymer approved by the FDA. It is biocompatible polymer with good mechanical properties and currently is a good alternative for tissue engineering after either chemical or morphological modifications.1, 2 In this regard, PCL is suitable for manufacturing porous templates by using different processing techniques such as salt
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leaching, phase separation, electrospinning, etc. The electrospinning is a simple and promising technique for making templates, which ca lead to microstructures similar to fibers in the extracellular matrix. This in turn, allows cell adhesion and promote tissue regeneration.3, 4 With this in mind, PCL templates were prepared by electrospinning and seeded with dental pulp stem cells (DPSC) with a potential application in bone regeneration. 5, 6, 7
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