Marine Genomics Methods and Protocols
This volume presents the latest protocols for both laboratory and bioinformatics based analyses in the field of marine genomics. The chapters presented in the book cover a wide range of topics, including the sampling and genomics of bacterial communities,
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Sarah J. Bourlat Editor
Marine Genomics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Marine Genomics Methods and Protocols
Edited by
Sarah J. Bourlat Department of Marine Sciences, University of Gothenburg, Gothenburg, Sweden
Editor Sarah J. Bourlat Department of Marine Sciences University of Gothenburg Gothenburg, Sweden
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3772-1 ISBN 978-1-4939-3774-5 (eBook) DOI 10.1007/978-1-4939-3774-5 Library of Congress Control Number: 2016943834 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover caption: Ephyra larva of the barrel jellyfish, Rhizostoma pulmo captured in a zooplankton haul off the Swedish west coast. – Photo By: Erik Selander, Department of Marine Sciences, University of Gothenburg, Sweden Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Marine genomics includes all aspects of genes and genomes of marine organisms aimed at understanding their evolution, function, biodiversity and environmental interactions. The field of genomics has been revolutionized by next generation sequencing, providing novel insights into one of the largest biomes on our planet. In this book, we present the latest protocols for both laboratory and bioinformatics based analyses in the field of marine genomics. The chapters presented here cover a wide range of topics, including the sampling and genomics of bacterial communities, DNA extraction in marine organisms, high-throughput sequencing of whole mitochondrial genomes, phylogenomics, SNP discovery, SNP arrays for species identification, digital PCR-based quantification methods, environmenta
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