Plant Gene Silencing Methods and Protocols
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene sil
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Kirankumar S. Mysore Muthappa Senthil-Kumar Editors
Plant Gene Silencing Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Gene Silencing Methods and Protocols
Edited by
Kirankumar S. Mysore Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA
Muthappa Senthil-Kumar National Institute of Plant Genome Research, New Delhi, India
Editors Kirankumar S. Mysore Plant Biology Division, The Samuel Roberts Noble Foundation Ardmore, OK, USA
Muthappa Senthil-Kumar National Institute of Plant Genome Research Aruna Asaf Ali Marg, New Delhi, India
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2452-3 ISBN 978-1-4939-2453-0 (eBook) DOI 10.1007/978-1-4939-2453-0 Library of Congress Control Number: 2015930614 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Gene silencing is being popularly used as a functional genomics tool to determine plant gene function. In addition, gene silencing is also used as one of the plant genetic engineering strategies to produce better crop varieties. It is anticipated that the use of gene silencing technology in commercial plant varieties will increase in the future. This volume will provide a comprehensive overview of various gene silencing methodologies and its applications. Gene silencing can be either transcriptional gene silencing (TGS) or posttranscriptional gene silencing (PTGS). PTGS is more popularly used for gene function analyses. PTGS is commonly achieved by either RNA interference (RNAi) or virus-induced gen
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