Plant Phosphoproteomics Methods and Protocols
This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in var
- PDF / 6,880,131 Bytes
- 234 Pages / 504.63 x 737.01 pts Page_size
- 17 Downloads / 240 Views
Waltraud X. Schulze Editor
Plant Phosphoproteomics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Phosphoproteomics Methods and Protocols Edited by
Waltraud X. Schulze Department of Plant Systems Biology, Universität Hohenheim, Stuttgart, Germany
Editor Waltraud X. Schulze Department of Plant Systems Biology Universität Hohenheim Stuttgart, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2647-3 ISBN 978-1-4939-2648-0 (eBook) DOI 10.1007/978-1-4939-2648-0 Library of Congress Control Number: 2015937355 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Phosphorylation is one of the most important posttranslational modifications involved in many regulatory processes within living cells. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein, and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The issue of Methods in Molecular Biology on “Plant Phosphoproteomics” addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system and is particularly designed as a protocol reference book compiled by leading experts in the field. Stuttgart, Germa
Data Loading...
