Porous scaffold made from collagen and liposomes coated with plant derived polysaccharide - in vivo study
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Porous scaffold made from collagen and liposomes coated with plant derived polysaccharide - in vivo study Otilia Zarnescu1, Lucia Moldovan2, Mihaela Trif3, Magdalena Moisei3, 2Alexandra Gaspar and Florin Leau4 1 University of Bucharest, Spl. Independentei 91-95, Bucharest, Romania. 2 National Institute of Research and Development for Biological Sciences, Splaiul Independentei 296, Bucharest, Romania. 3 Institute of Biochemistry, Splaiul Independentei 296, Bucharest, Romania. 4 Faculty of Veterinary Medicine, Splaiul Independentei 105, Bucharest, Romania.
ABSTRACT The purpose of this study was to investigate the in vivo biocompatibility of a new composite made from collagen and liposomes coated with plant derived polysaccharide using a rat subcutaneous implantation model. Histological observation has shown that after five days post-implantation, granulation tissue with vascular dilatation and inflammatory cell infiltration was formed adjacent to the implanted scaffold. After two weeks post-implantation, scaffold was highly infiltrated with cells and many of multinucleated giant cells were found to be aligned along the implant/tissue interface. At four weeks the scaffold was completely infiltrated with cells and a fibrous connective capsule isolate the implanted scaffold from the surrounding tissue. The inflammatory response to the implanted scaffold was assessed by immunohistochemical staining for interleukin 1β. IL-1β was demonstrated early in the post-implantation period (five days), but after two weeks, the intensity of IL-1β staining was on the decline. In vivo studies based on histological and immunohistochemical observations showed that throughout the period of implantation, composite scaffold was well tolerated by the host animals and immune responses to the implant were moderate. The observations are consistent with the normal wound healing response to subcutaneous implantation of biomaterials. The results from the in vivo study indicate a good biocompatibility for porous scaffold made from collagen and liposomes coated with plant derived polysaccharide. INTRODUCTION Lipid-based carrier systems, including liposomes and their nanoversions (nanoliposomes), are among the most promising encapsulation technologies employed in the rapidly developing field of nanotechnology [1]. Liposomes have been used to deliver active pharmacological substances (APS) into cells because they can entrap watersoluble (hydrophilic) APS in their internal water compartment and water-insoluble (hydrophobic) APS into the lipid bilayer [2]. Most of the work on the interactions between charged lipids and oppositely charged biopolymers has been restricted to association of liposomes with DNA [3], microtubules [4] glycosaminoglycans [5] and polysaccharides as pullulan, dextran, mannan and chitosan [6].
Clinical studies have shown that intra-articular administration of anti-inflammatory drugs encapsulated in liposomes shows prolonged residence in the joint and reduction of inflammation. Also, the anti-inflammatory properties of Urtica dioi
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