Oogenesis Methods and Protocols
This volume presents methods and techniques to study oogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guides readers through protocols on models of developmental biology, oogenesis in plants, worms, fruit f
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Ioannis P. Nezis Editor
Oogenesis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Oogenesis Methods and Protocols
Edited by
Ioannis P. Nezis School of Life Sciences, University of Warwick, Coventry, UK
Editor Ioannis P. Nezis School of Life Sciences University of Warwick Coventry, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3793-6 ISBN 978-1-4939-3795-0 (eBook) DOI 10.1007/978-1-4939-3795-0 Library of Congress Control Number: 2016946366 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Oogenesis is a key biological event in the life of multicellular organisms since its final product, the oocyte, is the absolute basis for the formation of the organism. Effective oogenesis is important for evolutionary success by transferring genetic information to the next generation and it is crucial for life. Hence, comprehension of molecular and cellular mechanisms that govern oogenesis is very critical for understanding of organismal physiology. In order to achieve this, scientists have developed a large variety of methods and techniques to study oogenesis. This book contains a collection of 20 chapters presenting methods and techniques to study oogenesis in a broad range of organisms, from plants to mammals. Specifically, the chapters include methods to study oogenesis in plants, worms, fruit flies, mosquitos, butterflies, starfish, zebrafish, frog, chicken, and mouse. The authors of the chapters are wellestablished and distinguished authorities in their respective areas of research. It was a great pleasure to edit this volume and c
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