Drosophila Oogenesis Methods and Protocols
This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene ex
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Diana P. Bratu Gerard P. McNeil Editors
Drosophila Oogenesis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Drosophila Oogenesis Methods and Protocols Edited by
Diana P. Bratu Department of Biological Sciences, Hunter College of the City University of New York, New York, NY, USA; Molecular, Cellular, and Developmental Biology Program, The Graduate Center, CUNY, New York, NY, USA
Gerard P. McNeil Department of Biology, School of Arts and Sciences, York College CUNY, Jamaica, NY, USA
Editors Diana P. Bratu Department of Biological Sciences Hunter College of the City University of New York New York, NY, USA
Gerard P. McNeil Department of Biology School of Arts and Sciences York College CUNY Jamaica, NY, USA
Molecular, Cellular, and Developmental Biology Program The Graduate Center, CUNY New York, NY, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2850-7 ISBN 978-1-4939-2851-4 (eBook) DOI 10.1007/978-1-4939-2851-4 Library of Congress Control Number: 2015944980 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Drosophila melanogaster is one of the most powerful model organisms in genetic studies of animal development and behavior. Entire tissues, from egg to adult, have been studied and continue to be the focus of cutting-edge biological research. With the accelerating pace of Drosophila genetics, the fruit fly will remain on the center stage for the foreseeable future. In this book we compiled a series of methods that have been developed to specifically address th
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